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低水平囊性纤维化跨膜传导调节因子（CFTR）促进子宫内膜癌的增殖和迁移。

Lower Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Promotes the Proliferation and Migration of Endometrial Carcinoma.

作者信息

Xia Xian, Wang Jie, Liu Yuan, Yue Ming

机构信息

Department of Nosocomial Infection Control, PLA Army General Hospital, Beijing, China (mainland).

School of Nursing, Nanjing Medical University, Nanjing, Jiangsu, China (mainland).

出版信息

Med Sci Monit. 2017 Feb 22;23:966-974. doi: 10.12659/msm.899341.

DOI:10.12659/msm.899341
PMID:28225751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5333717/
Abstract

BACKGROUND The incidence and death rates of endometrial cancer are alarmingly increasing. The diagnosis and treatment of endometrial cancer is crucial to decreasing mortality. Cystic fibrosis transmembrane conductance regulator (CFTR) belongs to the adenosine triphosphate (ATP)-binding cassette transporter family and plays an essential role in anion regulation and tissue homeostasis of various epithelia. This study explored the expression of CFTR in endometrial carcinoma and the role of CFTR in proliferation and migration of endometrial carcinoma cells. MATERIAL AND METHODS Immunohistochemistry and real-time (RT)-PCR were used to test the expression of CFTR in normal endometrium and endometrial carcinoma. CFTR inhibitor was used to restrain the expression of CFTR on the endometrial carcinoma, the effects on the proliferation and migration of endometrial carcinoma cells were also studied. RT-PCR was performed to test the expression of mir-125b after restraining CFTR. Proliferation and migration capability of endometrial carcinoma cells were detected after transfection of endometrial carcinoma cells with mir-125b mimic. RESULTS Compared with cells from normal endometrium, the expression of CFTR was significantly upregulated in endometrial carcinoma cells. After adding CFTR(inh)172, the capability for proliferation and transfer of endometrial carcinoma cells was strengthened, the expression of mir-125b was reduced, and after transfection with mir-125b mimics entering the endometrial carcinoma cells, the ability of the proliferation and transfer of endometrial carcinoma cells was also reduced. CONCLUSIONS The high expression of CFTR in the endometrial carcinoma cells played a pivotal role in restraining the proliferation and transfer of endometrial carcinoma cells.

摘要

背景

子宫内膜癌的发病率和死亡率正急剧上升。子宫内膜癌的诊断和治疗对于降低死亡率至关重要。囊性纤维化跨膜传导调节因子（CFTR）属于三磷酸腺苷（ATP）结合盒转运体家族，在各种上皮细胞的阴离子调节和组织稳态中起重要作用。本研究探讨了CFTR在子宫内膜癌中的表达及其在子宫内膜癌细胞增殖和迁移中的作用。

材料与方法

采用免疫组织化学和实时（RT）-PCR检测CFTR在正常子宫内膜和子宫内膜癌中的表达。使用CFTR抑制剂抑制子宫内膜癌中CFTR的表达，并研究其对子宫内膜癌细胞增殖和迁移的影响。抑制CFTR后，进行RT-PCR检测mir-125b的表达。用mir-125b模拟物转染子宫内膜癌细胞后，检测子宫内膜癌细胞的增殖和迁移能力。

结果

与正常子宫内膜细胞相比，子宫内膜癌细胞中CFTR的表达显著上调。加入CFTR(inh)172后，子宫内膜癌细胞的增殖和转移能力增强，mir-125b的表达降低，用mir-125b模拟物转染子宫内膜癌细胞后，子宫内膜癌细胞的增殖和转移能力也降低。

结论

CFTR在子宫内膜癌细胞中的高表达在抑制子宫内膜癌细胞的增殖和转移中起关键作用。

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