Miura T, Tsujimoto H, Fukasawa M, Kodama T, Shibuya M, Hasegawa A, Hayami M
Department of Animal Pathology, University of Tokyo, Japan.
Int J Cancer. 1987 Oct 15;40(4):564-9. doi: 10.1002/ijc.2910400422.
Tumor cells of feline leukemia were examined for structural abnormality and expression of the myc gene. Four of 12 spontaneous leukemia cases showed structural abnormalities of the myc gene detected by Southern blotting analysis. Of these 4 cases, one (Case 1) showed a DNA rearrangement in the 5' portion of the c-myc locus associated with integration of feline leukemia virus (FeLV) genome. Another case had a newly inserted myc gene which was transduced by FeLV. In the 2 other cases, no relation of abnormalities of the myc gene to FeLV was detected. One case of experimental leukemia induced by inoculation of a cell line, FT-1, established from Case 1 showed a c-myc gene rearrangement similar to that observed in Case 1. Expression of myc RNA was examined in 3 leukemia T-cell lines, FT-1, FL74 and F422, by Northern blotting analysis. A high level of myc transcript was detected in all 3 cell lines. The size of myc RNAs in FT-1 and FL74 was the same as that of normal myc RNA, while in F422 which had myc-containing FeLV (myc-FelV), the full genome and spliced form of myc-FeLV viral RNA were detected.
对猫白血病的肿瘤细胞进行了结构异常和myc基因表达的检测。在12例自发性白血病病例中，有4例通过Southern印迹分析检测到myc基因的结构异常。在这4例病例中，1例（病例1）在c-myc基因座的5'部分出现DNA重排，与猫白血病病毒（FeLV）基因组整合有关。另一例有一个新插入的由FeLV转导的myc基因。在另外2例病例中，未检测到myc基因异常与FeLV之间的关联。通过接种从病例1建立的细胞系FT-1诱导的1例实验性白血病，显示出与病例1中观察到的类似的c-myc基因重排。通过Northern印迹分析在3种白血病T细胞系FT-1、FL74和F422中检测了myc RNA的表达。在所有3种细胞系中均检测到高水平的myc转录本。FT-1和FL74中myc RNA的大小与正常myc RNA相同，而在含有myc的FeLV（myc-FelV）的F422中，检测到myc-FelV病毒RNA的全基因组和剪接形式。
