Obayemi John D, Hu Jingjie, Uzonwanne Vanessa O, Odusanya Olushola S, Malatesta Karen, Anuku Nicolas, Soboyejo Winston O
Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja, Federal Capital Territory, Nigeria; Department of Mechanical and Aerospace Engineering, Princeton University, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, NJ 08544, USA.
Department of Mechanical and Aerospace Engineering, Princeton University, NJ 08544, USA; Princeton Institute of Science and Technology of Materials (PRISM), Princeton, NJ 08544, USA.
J Mech Behav Biomed Mater. 2017 Apr;68:276-286. doi: 10.1016/j.jmbbm.2017.02.004. Epub 2017 Feb 6.
This paper presents the results of an experimental study of the adhesion forces between components of model conjugated magnetite nanoparticle systems for improved selectivity in the specific targeting of triple negative breast cancer. Adhesion forces between chemically synthesized magnetite nanoparticles (CMNPs), biosynthesized magnetite nanoparticles (BMNPs), as well as their conjugated systems and triple negative breast cancer cells (MDA-MB-231) or normal breast cells (MCF 10A) are elucidated at a nanoscale. In all cases, the BMNPs had higher adhesion forces (to breast cancer cells and normal breast cells) than CMNPs. The adhesion of LHRH-conjugated BMNPs or BSA-conjugated BMNPs to cancer cells is shown to be about 6 times to that of normal breast cells. The increase in adhesion forces between luteinizing hormone-releasing hormone, LHRH- or EphA2, a breast specific antibody(BSA)-conjugated BMNPs to breast cancer cells is attributed to van der Waals interactions between the peptides/antibodies from the conjugated nanoparticles and the over-expressed receptors (revealed using immunofluorescence staining) on the surfaces of the breast cancer. The implications of the results are discussed for the selectivity and specificity of breast cancer targeting by ligand-conjugated BMNPs.
本文介绍了一项实验研究的结果，该研究针对模型共轭磁铁矿纳米颗粒系统的各组分之间的粘附力展开，以提高三阴性乳腺癌特异性靶向的选择性。在纳米尺度上阐明了化学合成磁铁矿纳米颗粒（CMNP）、生物合成磁铁矿纳米颗粒（BMNP）及其共轭系统与三阴性乳腺癌细胞（MDA-MB-231）或正常乳腺细胞（MCF 10A）之间的粘附力。在所有情况下，BMNP（对乳腺癌细胞和正常乳腺细胞）的粘附力均高于CMNP。结果表明，促黄体激素释放激素（LHRH）共轭的BMNP或牛血清白蛋白（BSA）共轭的BMNP对癌细胞的粘附力约为对正常乳腺细胞的6倍。促黄体激素释放激素（LHRH）或EphA2（一种乳腺特异性抗体（BSA））共轭的BMNP与乳腺癌细胞之间粘附力的增加归因于共轭纳米颗粒中的肽/抗体与乳腺癌表面过表达受体（通过免疫荧光染色显示）之间的范德华相互作用。文中讨论了这些结果对配体共轭BMNP靶向乳腺癌的选择性和特异性的意义。
