Department of Materials Science and Engineering, African University of Science and Technology (AUST) Abuja-Nigeria, Abuja, Nigeria.
Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO) P.M.B 186, Garki, Abuja, Nigeria.
J Mater Sci Mater Med. 2017 Aug 17;28(9):143. doi: 10.1007/s10856-017-5943-2.
This paper explores the adhesion of biosynthesized gold nanoparticles (AuNPs) and gold (Au) nanoparticle/prodigiosin (PG) drug nanoparticles to breast cancer cells (MDA-MB-231 cells). The AuNPs were synthesized in a record time (less than 30 s) from Nauclea latifolia leaf extracts, while the PG was produced via bacterial synthesis with Serratia marcescens sp. The size distributions and shapes of the resulting AuNPs were characterized using transmission electron microscopy (TEM), while the resulting hydrodynamic diameters and polydispersity indices were studied using dynamic light scattering (DLS). Atomic Force Microscopy (AFM) was used to study the adhesion between the synthesized gold nanoparticles (AuNPs)/LHRH-conjugated AuNPs and triple negative breast cancer cells (MDA-MB-231 cells), as well as the adhesion between LHRH-conjugated AuNP/PG drug and MDA-MB-231 breast cancer cells. The adhesion forces between LHRH-conjugated AuNPs and breast cancer cells are shown to be five times greater than those between AuNPs and normal breast cells. The increase in adhesion is shown to be due to the over-expression of LHRH receptors on the surfaces of MDA-MB-231 breast cancer cells, which was revealed by confocal immuno-fluorescence microscopy. The implications of the results are then discussed for the selective and specific targeting and treatment of triple negative breast cancer.
本文探讨了生物合成的金纳米粒子（AuNPs）和金（Au）纳米粒子/灵菌红素（PG）药物纳米粒子与乳腺癌细胞（MDA-MB-231 细胞）的黏附作用。AuNPs 是在短至 30 秒的时间内从乌檀树叶提取物中合成的，而 PG 是通过细菌合成的，所用细菌为粘质沙雷氏菌（Serratia marcescens sp.）。用透射电子显微镜（TEM）对所得 AuNPs 的尺寸分布和形状进行了表征，而用动态光散射（DLS）研究了所得 AuNPs 的水动力直径和多分散指数。原子力显微镜（AFM）用于研究合成的金纳米粒子（AuNPs）/LHRH 缀合的 AuNPs 与三阴性乳腺癌细胞（MDA-MB-231 细胞）之间的黏附，以及 LHRH 缀合的 AuNP/PG 药物与 MDA-MB-231 乳腺癌细胞之间的黏附。结果表明，LHRH 缀合的 AuNPs 与乳腺癌细胞之间的黏附力是 AuNPs 与正常乳腺细胞之间的 5 倍。黏附力的增加是由于 MDA-MB-231 乳腺癌细胞表面 LHRH 受体的过度表达，这一点通过共聚焦免疫荧光显微镜得到了揭示。然后，讨论了这些结果对三阴性乳腺癌的选择性和特异性靶向治疗的意义。
