人髓质素互补DNA的分子克隆：一种骨髓细胞中的炎性丝氨酸蛋白酶。

Molecular cloning of complementary DNA for human medullasin: an inflammatory serine protease in bone marrow cells.

作者信息

Okano K, Aoki Y, Sakurai T, Kajitani M, Kanai S, Shimazu T, Shimizu H, Naruto M

机构信息

Basic Research Laboratories, Toray Industries, Inc., Kanagawa.

出版信息

J Biochem. 1987 Jul;102(1):13-6. doi: 10.1093/oxfordjournals.jbchem.a122024.

DOI:10.1093/oxfordjournals.jbchem.a122024

PMID:2822677

Abstract

Medullasin, an inflammatory serine protease in bone marrow cells, modifies the functions of natural killer cells, monocytes, and granulocytes. We have cloned a medullasin cDNA from a human acute promyelocytic cell (ML3) cDNA library using oligonucleotide probes synthesized from the information of N-terminal amino acid sequence of natural medullasin. The cDNA contained a long open reading frame encoding 237 amino acid residues beginning from the second amino acid of natural meduallasin. The deduced amino acid sequence of medullasin shows a typical serine protease structure, with 41% homology with pig elastase 1.

摘要

髓素是骨髓细胞中的一种炎性丝氨酸蛋白酶，可改变自然杀伤细胞、单核细胞和粒细胞的功能。我们利用根据天然髓素N端氨基酸序列信息合成的寡核苷酸探针，从人急性早幼粒细胞（ML3）cDNA文库中克隆出了髓素cDNA。该cDNA包含一个长开放阅读框，从天然髓素的第二个氨基酸开始编码237个氨基酸残基。髓素推导的氨基酸序列显示出典型的丝氨酸蛋白酶结构，与猪弹性蛋白酶1有41%的同源性。