Franklyn J A, Wood D F, Balfour N J, Ramsden D B, Docherty K, Sheppard M C
Department of Endocrinology, University of Birmingham, Queen Elizabeth Hospital, Edgbaston.
J Endocrinol. 1987 Oct;115(1):53-9. doi: 10.1677/joe.0.1150053.
The role of oestrogen in the regulation of TSH gene expression is unclear. We have examined the effect of administration of oestrogen in the rat on serum TSH, pituitary TSH content and pituitary cytoplasmic concentrations of mRNA encoding the TSH beta and alpha subunits, thus deriving measures of hormone release and synthesis. In addition, we have examined the effect of oestrogen on the binding of tri-iodothyronine (T3) to nuclear receptors in the anterior pituitary. Administration of oestrogen did not affect serum concentrations of TSH in euthyroid or untreated hypothyroid rats, but did augment the effects of T3 (1 and 2 micrograms) on serum TSH in hypothyroid animals 6 h after injection of T3. No influence of oestrogen or of thyroid status on pituitary content of TSH was seen. A marked increase in the concentrations of TSH beta and alpha mRNA in pituitary cytoplasm was found in hypothyroidism, compared with those in the euthyroid state. No effect of oestrogen on TSH mRNA was seen in euthyroid animals but concentrations of TSH beta and alpha mRNA were lower in hypothyroid animals than in vehicle-treated controls. A stimulatory influence of T3 on TSH mRNA was seen 6 h after injection of T3; this stimulation was absent in oestrogen-treated rats. No effect of oestrogen on the action of T3 was evident 72 h after beginning treatment with T3. In addition to effects on serum TSH and TSH mRNA, an increase in the number of pituitary nuclear receptors for T3 was seen after oestrogen treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
雌激素在促甲状腺激素（TSH）基因表达调控中的作用尚不清楚。我们研究了给大鼠注射雌激素对血清TSH、垂体TSH含量以及垂体细胞质中编码TSHβ和α亚基的mRNA浓度的影响，从而得出激素释放和合成的指标。此外，我们还研究了雌激素对垂体前叶三碘甲状腺原氨酸（T3）与核受体结合的影响。给甲状腺功能正常或未经治疗的甲状腺功能减退大鼠注射雌激素，对血清TSH浓度没有影响，但在给甲状腺功能减退动物注射T3 6小时后，雌激素确实增强了T3（1微克和2微克）对血清TSH的作用。未观察到雌激素或甲状腺状态对垂体TSH含量有影响。与甲状腺功能正常状态相比，甲状腺功能减退时垂体细胞质中TSHβ和α mRNA的浓度显著增加。在甲状腺功能正常的动物中未观察到雌激素对TSH mRNA有影响，但在甲状腺功能减退的动物中，TSHβ和α mRNA的浓度低于用赋形剂处理的对照组。注射T3 6小时后，观察到T3对TSH mRNA有刺激作用；在用雌激素处理的大鼠中这种刺激作用不存在。在用T3开始治疗72小时后，未发现雌激素对T3的作用有明显影响。除了对血清TSH和TSH mRNA有影响外，雌激素处理后还观察到垂体T3核受体数量增加。（摘要截短于250字）
