Chiu Chih-Hao, Liu Jun-Liang, Chang Chih-Hsuan, Lei Kin Fong, Chen Alvin Chao-Yu
Bone and Joint Research Center, Department of Orthopedic Surgery, Chang Gung Memorial Hospital, Tooyuan Branch, Taoyuan, Taiwan.
Graduate Institute of Medical Mechatronics, Chang Gung University, Taoyuan, Taiwan.
Biomed Microdevices. 2017 Mar;19(1):13. doi: 10.1007/s10544-017-0154-7.
Periosteum-derived cells was indicated to respond to mechanical force and have stem cell potential capable of differentiating into multiple tissue. Investigation of osteogenic activity under mechanical stimulation is important to understand the therapeutic conditions of fracture healing. In this work, a cell culture platform was developed for respectively providing isotropic and anisotropic axial strain. Primary rabbit periosteal cells were isolated and cultured in the chamber. Multi-axial tensile strain was received and osteogenic activity was investigated by mRNA expressions of CBFA1 and OPN. The highest mRNA expression was found in moderate strain (5-8%) under anisotropic axial strain. These results provided important foundation for further in vivo studies and development of tailor-made stretching rehabilitation equipment.
骨膜来源的细胞被证明对机械力有反应，并且具有能够分化为多种组织的干细胞潜能。研究机械刺激下的成骨活性对于理解骨折愈合的治疗条件很重要。在这项工作中，开发了一种细胞培养平台，用于分别提供各向同性和各向异性轴向应变。分离原代兔骨膜细胞并在培养室中培养。施加多轴拉伸应变，并通过CBFA1和OPN的mRNA表达研究成骨活性。在各向异性轴向应变下的中等应变（5-8%）中发现了最高的mRNA表达。这些结果为进一步的体内研究和定制拉伸康复设备的开发提供了重要基础。
