Kawashima Hideki, Ohnishi Masao
Bioscience Laboratory, Miyako College, Iwate Prefectural University, 1-5-1 Kanan, Miyako, Iwate, 027-0031, Japan.
Department of Food Science and Human Nutrition, Faculty of Human Life Sciences, Fuji Women's University, Ishikari, 061-3204, Japan.
Lipids. 2017 Apr;52(4):375-381. doi: 10.1007/s11745-017-4240-7. Epub 2017 Feb 23.
Our previous study characterized highly diverse dienoic fatty acids (FA), in particular an uncommon non-methylene-interrupted (NMI) FA, in the ovaries of the Japanese limpet Cellana toreuma belonging to the archaeogastropods, but many minor chemically unidentified FA remain. In this study, among previously unidentified FA (less than 0.1% of total FA), four novel NMI FA with a terminal double bond [7,18-nonadecadienoic (19:2Δ7,18), 11,18-nonadecadienoic (19:2Δ11,18), 7,20-heneicosadienoic (21:2Δ7,20), and 11,20-heneicosadienoic (21:2Δ11,20) acids] were found, along with known 14-pentadecenoic (15:1Δ14), 16-heptadecenoic (17:1Δ16), and 9,18-nonadecadienoic (19:2Δ9,18) acids, based on capillary GC-MS of their methyl esters, 3-pyridylcarbinol derivatives, and argentation thin-layer chromatography. From our findings, possible biosynthetic pathways for the novel FA are discussed.
我们之前的研究对属于原始腹足类的日本笠贝（Cellana toreuma）卵巢中高度多样的二烯脂肪酸（FA）进行了表征，特别是一种罕见的非亚甲基中断（NMI）脂肪酸，但仍有许多化学性质未明确的微量FA。在本研究中，基于甲酯的毛细管气相色谱-质谱联用（GC-MS）、3-吡啶甲醇衍生物以及银化薄层色谱法，在先前未鉴定的FA（占总FA的比例小于0.1%）中，发现了四种带有末端双键的新型NMI FA [7,18-十九碳二烯酸（19:2Δ7,18）、11,18-十九碳二烯酸（19:2Δ11,18）、7,20-二十一碳二烯酸（21:2Δ7,20）和11,20-二十一碳二烯酸（21:2Δ11,20）]，以及已知的14-十五碳烯酸（15:1Δ14）、16-十七碳烯酸（17:1Δ16）和9,18-十九碳二烯酸（19:2Δ9,18）。根据我们的研究结果，讨论了新型FA可能的生物合成途径。
