Ueno T, Suzuki N, Kaneko A, Fujinaga K
Department of Ophthalmology, Sapporo Medical College, Japan.
Jpn J Ophthalmol. 1987;31(2):274-83.
Human adenovirus groups A and B have an oncogenic potential in newborn rodents. Especially, adenovirus type 12 is known to induce retinoblastoma-like tumor in baboons and transform human embryo retinoblast cells in vitro. Human JC virus is also known to produce a variety of tumors in newborn rodents, including retinoblastoma-like tumor. In this experiment, cell DNAs of human retinoblastomas were assayed for each of the transforming gene sequences of adenovirus groups A, B, C, D and E and for JC virus gene sequences, by using Southern blot hybridization. None of these viral gene sequences were detected at the level of 0.1-0.5 copy per diploid cell DNA in all of 11 retinoblastomas, including 6 retinoblastomas previously examined for adenovirus 12-transforming gene sequences. This led to a conclusion that most, if not all, adenoviruses and JC virus play no essential role in the etiology of human retinoblastoma, although there were experimental models of retinoblastoma induced by these viruses.
人类A组和B组腺病毒对新生啮齿动物具有致癌潜力。特别是，已知12型腺病毒可在狒狒中诱发视网膜母细胞瘤样肿瘤，并在体外转化人胚胎视网膜母细胞。人类JC病毒也已知在新生啮齿动物中产生多种肿瘤，包括视网膜母细胞瘤样肿瘤。在本实验中，通过Southern印迹杂交法检测了人类视网膜母细胞瘤的细胞DNA中A、B、C、D和E组腺病毒的每种转化基因序列以及JC病毒基因序列。在包括6例先前检测过12型腺病毒转化基因序列的视网膜母细胞瘤在内的所有11例视网膜母细胞瘤中，均未在每个二倍体细胞DNA 0.1 - 0.5拷贝的水平检测到这些病毒基因序列。这得出一个结论，尽管存在这些病毒诱发视网膜母细胞瘤的实验模型，但大多数（如果不是全部）腺病毒和JC病毒在人类视网膜母细胞瘤的病因学中不发挥重要作用。
