Suzuki N, Ueno T, Kaneko A, Fujii S, Fujinaga K
Graefes Arch Clin Exp Ophthalmol. 1983;220(4):167-70. doi: 10.1007/BF02186662.
Adenovirus type 12 has high oncogenic potential in newborn rodents. Moreover, adenovirus 12 induces retinoblastoma-like tumours in baboons and transforms in vitro human embryo retinoblasts. Since adenovirus-transformed cells contain adenovirus transforming gene sequences, the detection of adenovirus 12 transforming gene in tumour cell DNA can provide evidence for or against a possible aetiological role of adenovirus 12 in retinoblastoma. In this experiment, cell DNAs from six retinoblastomas were assayed for adenovirus 12 transforming gene sequences by spot hybridization and Southern blot hybridization, using the labelled EcoRI-C fragment of adenovirus 12 DNA as a probe (the far left 16.5% of the viral genome). No adenovirus 12 transforming gene sequences were detected at the level of 0.1 or 0.5 copy of the probe per diploid cell DNA in all of six retinoblastomas.
12型腺病毒在新生啮齿动物中具有很高的致癌潜力。此外，12型腺病毒可在狒狒中诱发视网膜母细胞瘤样肿瘤，并在体外转化人胚胎视网膜母细胞。由于腺病毒转化细胞含有腺病毒转化基因序列，因此检测肿瘤细胞DNA中的12型腺病毒转化基因可为12型腺病毒在视网膜母细胞瘤中可能的病因学作用提供支持或反对的证据。在本实验中，使用12型腺病毒DNA的标记EcoRI-C片段（病毒基因组最左侧的16.5%）作为探针，通过斑点杂交和Southern印迹杂交检测了六个视网膜母细胞瘤的细胞DNA中12型腺病毒转化基因序列。在所有六个视网膜母细胞瘤中，未检测到每二倍体细胞DNA中探针水平为0.1或0.5拷贝的12型腺病毒转化基因序列。
