Schmidt Marcel, Toplak Ana, Quaedflieg Peter Jlm, Nuijens Timo
EnzyPep B.V., Urmonderbaan 22, 6167RD Geleen, The Netherlands; Van't Hoff Institute of Molecular Sciences, University of Amsterdam, Science Park 904, 1098XH Amsterdam, The Netherlands.
EnzyPep B.V., Urmonderbaan 22, 6167RD Geleen, The Netherlands.
Curr Opin Chem Biol. 2017 Jun;38:1-7. doi: 10.1016/j.cbpa.2017.01.017. Epub 2017 Apr 20.
With the steadily increasing complexity and quantity requirements for peptides in industry and academia, the efficient and site-selective ligation of peptides and proteins represents a highly desirable goal. Within this context, enzyme-mediated ligation technologies for peptides and proteins have attracted great interest in recent years as they represent an extremely powerful extension to the scope of chemical methodologies (e.g. native chemical ligation) in basic and applied research. Compared to chemical ligation methods, enzymatic strategies using ligases such as sortase, butelase, peptiligase or omniligase generally feature excellent chemoselectivity, therefore making them valuable tools for protein and peptide chemists.
随着工业和学术界对肽的复杂性和数量需求稳步增加，肽与蛋白质的高效且位点选择性连接成为一个极具吸引力的目标。在此背景下，近年来用于肽和蛋白质的酶介导连接技术引起了极大关注，因为它们是基础研究和应用研究中化学方法（如天然化学连接）范围的极其有力的扩展。与化学连接方法相比，使用诸如分选酶、丁酯酶、肽连接酶或全能连接酶等连接酶的酶促策略通常具有出色的化学选择性，因此使其成为蛋白质和肽化学家的宝贵工具。
