Revuelta J L, Jayaram M
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Mol Cell Biol. 1987 Aug;7(8):2664-70. doi: 10.1128/mcb.7.8.2664-2670.1987.
We have cloned the gene encoding the TRPF and TRPC functions of Phycomyces blakesleeanus by complementation of the corresponding activities of Escherichia coli. TRPF also complemented a trpl mutation in Saccharomyces cerevisiae. As in other filamentous fungi, such as Neurospora and Aspergillus spp., the P. blakesleeanus TRPF and TRPC formed part of a trifunctional polypeptide encoded by a single gene (called TRP1). Transcription of TRP1 in P. blakesleeanus did not appear to be regulated by light or by the nutritional status of the culture. The information on the structure and organization of a P. blakesleeanus gene derived from these studies should be useful in devising molecular genetic strategies to analyze the sensory physiology of this organism.
我们通过补充大肠杆菌的相应活性，克隆了编码布氏毛霉TRPF和TRPC功能的基因。TRPF也补充了酿酒酵母中的trp1突变。与其他丝状真菌（如粗糙脉孢菌和曲霉属物种）一样，布氏毛霉的TRPF和TRPC是由单个基因（称为TRP1）编码的三功能多肽的一部分。布氏毛霉中TRP1的转录似乎不受光照或培养物营养状况的调节。从这些研究中获得的关于布氏毛霉基因结构和组织的信息，对于设计分子遗传学策略来分析该生物体的感觉生理学应该是有用的。
