Kos A, Kuijvenhoven J, Wernars K, Bos C J, van den Broek H W, Pouwels P H, van den Hondel C A
Gene. 1985;39(2-3):231-8. doi: 10.1016/0378-1119(85)90317-8.
The Aspergillus niger trpC gene was isolated by complementation experiments with an Escherichia coli trpC mutant. Plasmid DNA containing the A. niger trpC gene transforms an Aspergillus nidulans mutant strain, defective in all three enzymatic activities of the trpC gene, to Trp+, indicating the presence of a complete and functional trpC gene. Southern blot analysis of DNA from these Trp+ transformants showed that plasmid DNA was present but that this DNA was not integrated at the site of the chromosomal trpC locus. The A. niger trpC gene was localized on the cloned fragment by heterologous hybridization experiments and sequence analysis. These experiments suggest that the organization of the A. niger trpC gene is identical to that of the analogous A. nidulans trpC and the Neurospora crassa trp-1 genes.
通过与大肠杆菌trpC突变体进行互补实验，分离出了黑曲霉trpC基因。含有黑曲霉trpC基因的质粒DNA将在trpC基因的所有三种酶活性方面存在缺陷的构巢曲霉突变株转化为Trp+，这表明存在完整且有功能的trpC基因。对这些Trp+转化体的DNA进行Southern印迹分析表明，存在质粒DNA，但该DNA未整合到染色体trpC基因座位点。通过异源杂交实验和序列分析，将黑曲霉trpC基因定位在克隆片段上。这些实验表明，黑曲霉trpC基因的组织方式与构巢曲霉类似的trpC基因以及粗糙脉孢菌trp-1基因相同。
