来自人类细胞的一种ATP依赖性DNA链转移酶的特性分析。
Characterization of an ATP-dependent DNA strand transferase from human cells.
作者信息
Ganea D, Moore P, Chekuri L, Kucherlapati R
机构信息
Center for Genetics, University of Illinois College of Medicine, Chicago 60612.
出版信息
Mol Cell Biol. 1987 Sep;7(9):3124-30. doi: 10.1128/mcb.7.9.3124-3130.1987.
Abstract
We have characterized an enzymatic activity from human cell nuclei which is capable of catalyzing strand exchange between homologous DNA sequences. The strand exchange activity was Mg2+ dependent and required ATP hydrolysis. In addition, it was capable of promoting reannealing of homologous DNA sequences and could form nucleoprotein networks in a fashion reminiscent of purified bacterial RecA protein. Using an in vitro recombination assay, we also showed that the strand exchange activity was biologically important. The factor(s) responsible for the activity has been partially purified.
摘要
我们已经鉴定了一种来自人类细胞核的酶活性，它能够催化同源DNA序列之间的链交换。链交换活性依赖于Mg2+，并且需要ATP水解。此外，它能够促进同源DNA序列的重新退火，并且能够以类似于纯化的细菌RecA蛋白的方式形成核蛋白网络。使用体外重组试验，我们还表明链交换活性在生物学上是重要的。负责该活性的因子已被部分纯化。
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1
Improved method for obtaining thymineless mutants of Escherichia coli and Salmonella typhimurium.获得大肠杆菌和鼠伤寒沙门氏菌无胸腺嘧啶突变体的改进方法。
J Bacteriol. 1962 Sep;84(3):602-3. doi: 10.1128/jb.84.3.602-603.1962.
2
Concerted strand exchange and formation of Holliday structures by E. coli RecA protein.大肠杆菌RecA蛋白协同进行的链交换及霍利迪结构的形成
Cell. 1981 Aug;25(2):507-16. doi: 10.1016/0092-8674(81)90069-6.
3
Hydrolysis of nucleoside triphosphates catalyzed by the recA protein of Escherichia coli. Characterization of ATP hydrolysis.大肠杆菌recA蛋白催化的核苷三磷酸水解。ATP水解的特性
J Biol Chem. 1981 Aug 25;256(16):8829-34.
4
Heteroduplex formation and polarity during strand transfer promoted by Ustilago rec 1 protein.黑粉菌rec 1蛋白促进链转移过程中的异源双链体形成和极性。
Cell. 1983 Jul;33(3):857-64. doi: 10.1016/0092-8674(83)90028-4.
5
Genetic recombination of homologous plasmids catalyzed by cell-free extracts of Saccharomyces cerevisiae.酿酒酵母无细胞提取物催化的同源质粒基因重组
Cell. 1983 Dec;35(3 Pt 2):805-13. doi: 10.1016/0092-8674(83)90113-7.
6
Homologous recombination catalyzed by mammalian cell extracts in vitro.体外由哺乳动物细胞提取物催化的同源重组。
Science. 1984 Dec 7;226(4679):1213-5. doi: 10.1126/science.6334360.
7
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.DNA转入小鼠L细胞过程中的同源重组模型：DNA末端在重组过程中的作用。
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8
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9
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Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
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