Cassuto E, Lightfoot L A, Howard-Flanders P
Mol Gen Genet. 1987 Jun;208(1-2):10-4. doi: 10.1007/BF00330415.
An activity that can promote homologous pairing and strand transfer between suitable DNA substrates has been partially purified from human skin fibroblasts and from HeLa cells. The strand transfer reaction was investigated with DNA substrates consisting of single-stranded circular and duplex linear phage DNA. It requires ATP, and under optimal conditions yields heteroduplex molecules containing one strand from each parental DNA substrate. The reactions appears to be of the same general nature as those mediated by RecA proteins of Escherichia coli and the Rec1 protein of Ustilago maydis.
一种能够促进合适的DNA底物之间进行同源配对和链转移的活性物质已从人皮肤成纤维细胞和HeLa细胞中得到部分纯化。利用由单链环状和双链线性噬菌体DNA组成的DNA底物对链转移反应进行了研究。该反应需要ATP，并且在最佳条件下可产生含有来自每个亲本DNA底物的一条链的异源双链分子。该反应似乎与大肠杆菌的RecA蛋白和玉米黑粉菌的Rec1蛋白介导的反应具有相同的一般性质。
