烟酰胺腺嘌呤二核苷酸（NADH）-延胡索酸还原酶在布氏锥虫超氧阴离子和过氧化氢产生中的可能作用。
Possible role of the NADH-fumarate reductase in superoxide anion and hydrogen peroxide production in Trypanosoma brucei.
作者信息
Turrens J F
机构信息
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD.
出版信息
Mol Biochem Parasitol. 1987 Aug;25(1):55-60. doi: 10.1016/0166-6851(87)90018-1.
Mitochondrial membranes from Trypanosoma brucei procyclic trypomastigotes generated superoxide anion and hydrogen peroxide in a 2:1 ratio when supplemented with NADH. Fumarate inhibited hydrogen peroxide formation (Ki = 16 microM) with the same affinity as it stimulated NADH-fumarate reductase activity. Superoxide anion production was also 65% inhibited by fumarate (Ki = 20 microM). The KM for NADH of the NADH-fumarate reductase (60 microM) was also similar to that for hydrogen peroxide generation in the absence of fumarate (30 microM). These results suggest that the NADH-fumarate reductase is involved as a source for free radical generation in T. brucei mitochondria.
当补充烟酰胺腺嘌呤二核苷酸（NADH）时，来自布氏锥虫前循环型锥鞭毛体的线粒体膜以2:1的比例产生超氧阴离子和过氧化氢。延胡索酸抑制过氧化氢的形成（抑制常数Ki = 16微摩尔），其亲和力与它刺激NADH-延胡索酸还原酶活性时相同。延胡索酸对超氧阴离子产生的抑制作用也达65%（抑制常数Ki = 20微摩尔）。NADH-延胡索酸还原酶对NADH的米氏常数（KM）（60微摩尔）也与在没有延胡索酸时过氧化氢生成的米氏常数（30微摩尔）相似。这些结果表明，NADH-延胡索酸还原酶参与了布氏锥虫线粒体中自由基产生的过程。
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