Walsh J W, Zimmer S G, Oeltgen J
Neurosurgical Service, Veterans Administration Medical Center, Lexington, Kentucky.
Neurosurgery. 1987 Sep;21(3):361-70. doi: 10.1227/00006123-198709000-00015.
We previously described an experimental model for the production of invasive and noninvasive astrocytic series tumors and a semiquantitative assessment of their invasiveness. The tumors are produced by intracerebral inoculation of simian virus 40-transformed cells from four different brain regions. Cells of cerebral cortex and brain stem derivation produced invasive tumors; tumors of cerebral cortex derivation were more invasive and became increasingly invasive with alternate in vivo and in vitro passage. Cells of cerebellar hemisphere and vermis derivation produced primarily noninvasive tumors. This report describes the surface configuration of these tumor cells in culture at or near confluence as visualized with scanning electron microscopy. Normal cells form a multilayered base of flat, overlapping cells with few excrescences and indistinct borders and have only rare dividing or giant cells attached. Invasive tumor cells from a monolayer base of extremely flat and spread out cells almost devoid of excrescences, with few dividing or giant cells attached. Cells of cerebral cortex derivation additionally have numerous microvilli and ruffles at points of intercellular contact and become covered with microvilli after passage. These features were retained after cloning by dilution plating, but not after cloning by growth in soft agar. Our primarily noninvasive cells form a multilayered base of rounded cells covered with various excrescences and numerous attached dividing and giant cells. These surface features seem to be related to intercellular and cell-substrate adherence. The configuration of the invasive cells is consistent with increased cell-substrate adherence, substrate-dependent inhibition of locomotion, and decreased intercellular adherence among the more invasive cells. The configuration of our primarily noninvasive cells is consistent with decreased cell-substrate adherence and unrestricted multilayered growth. Thus, it seems that invasiveness is expressed at the cell surface and is related to disturbances in adherence balance rather than in cell proliferation.
我们之前描述了一种用于产生侵袭性和非侵袭性星形细胞系列肿瘤的实验模型，以及对其侵袭性的半定量评估。这些肿瘤是通过将来自四个不同脑区的猿猴病毒40转化细胞脑内接种产生的。大脑皮质和脑干来源的细胞产生侵袭性肿瘤；大脑皮质来源的肿瘤侵袭性更强，并且随着体内和体外交替传代而侵袭性增加。小脑半球和蚓部来源的细胞主要产生非侵袭性肿瘤。本报告描述了用扫描电子显微镜观察到的这些肿瘤细胞在汇合或接近汇合时的培养表面形态。正常细胞形成多层扁平、重叠的细胞基底，几乎没有赘生物，边界不清晰，仅有罕见的分裂或巨细胞附着。侵袭性肿瘤细胞来自单层基底，细胞极其扁平且伸展，几乎没有赘生物，附着的分裂或巨细胞很少。大脑皮质来源的细胞在细胞间接触点还具有大量微绒毛和褶皱，传代后被微绒毛覆盖。这些特征在通过稀释平板克隆后得以保留，但在软琼脂中生长克隆后则没有。我们的主要非侵袭性细胞形成多层圆形细胞基底，覆盖着各种赘生物以及大量附着的分裂和巨细胞。这些表面特征似乎与细胞间和细胞与底物的黏附有关。侵袭性细胞的形态与细胞与底物黏附增加、底物依赖性运动抑制以及侵袭性更强的细胞间黏附减少一致。我们主要非侵袭性细胞的形态与细胞与底物黏附减少和不受限制的多层生长一致。因此，似乎侵袭性在细胞表面表达，并且与黏附平衡的紊乱而非细胞增殖有关。
