Walsh J W, Zimmer S G, Oeltgen J, Mason R, Perdue M L, Markesbery W R
J Neuropathol Exp Neurol. 1982 Nov;41(6):588-605. doi: 10.1097/00005072-198211000-00003.
Neoplastic transformation of one-day-old hamster brain cells was produced by infection with SV-40 virus and verified by phase contrast microscopy, growth in semisolid media, and intracranial tumor production after inoculation of the cells into other one-day-old hamsters. Transformed cells were studied by transmission and scanning electron microscopy. The numerous alterations in cell surface structure and in nuclear and cytoplasmic organization suggest a marked increase in cell metabolism and in the rate of mitosis and cell division. Cilia with a nine-to-zero pattern of microtubule doublets were present in cells with intermediate size filaments which stained for glial fibrillary acidic protein. The findings indicate that infection of one-day-old hamster brain cells in culture by SV-40 virus results in their transformation to a neoplastic state and the transformed cells are differentiating neoplastic astrocytes.
用SV - 40病毒感染一日龄仓鼠脑细胞可导致肿瘤转化，通过相差显微镜检查、在半固体培养基中的生长情况以及将细胞接种到其他一日龄仓鼠体内后的颅内肿瘤形成来进行验证。通过透射电子显微镜和扫描电子显微镜对转化细胞进行了研究。细胞表面结构以及细胞核和细胞质组织的大量改变表明细胞代谢、有丝分裂率和细胞分裂率显著增加。在具有中等大小细丝的细胞中存在微管二联体呈9 + 0模式的纤毛，这些细丝对胶质纤维酸性蛋白呈阳性染色。研究结果表明，培养的一日龄仓鼠脑细胞被SV - 40病毒感染会导致其转化为肿瘤状态，且转化细胞为正在分化的肿瘤性星形胶质细胞。
