Pan Changkun, Wang Wei, Yuan Huifang, Yang Lirong, Chen Baoru, Li Dengfeng, Chen Jianming
School of Marine Sciences, Ningbo University, Ningbo 315211, Zhejiang, China; State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China.
State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China.
Dev Comp Immunol. 2017 Jul;72:89-96. doi: 10.1016/j.dci.2017.02.012. Epub 2017 Feb 20.
The world production of shrimp is seriously affected by the white spot syndrome virus (WSSV). Viral immediate-early (IE) genes encode regulatory proteins critical for the viral lifecycle. In spite of their importance, only five out of the 21 identified WSSV IE genes are functionally characterized. Here, we report the use of Drosophila melanogaster as a model to explore the role of WSSV IE gene wsv187. In vivo expression of WSV187 in transgenic flies show WSV187 localized in the cytoplasm. Overexpression of wsv187 results wing defects consistent with phenotypes observed in JAK/STAT exacerbated flies. After artificial infection of the DCV virus, the flies expressing wsv187 showed a lower viral load, a higher survival rate and an up-regulated STAT92E expression. These data demonstrate wsv187 plays a role in the controlling of virus replication by activating host JAK/STAT pathway.
全球虾类产量受到白斑综合征病毒（WSSV）的严重影响。病毒即刻早期（IE）基因编码对病毒生命周期至关重要的调节蛋白。尽管它们很重要，但在已鉴定的21个WSSV IE基因中，只有5个具有功能特征。在此，我们报告利用黑腹果蝇作为模型来探究WSSV IE基因wsv187的作用。WSV187在转基因果蝇中的体内表达显示其定位于细胞质中。wsv187的过表达导致翅膀缺陷，这与在JAK/STAT信号通路增强的果蝇中观察到的表型一致。在人工感染果蝇C病毒（DCV）后，表达wsv187的果蝇显示出较低的病毒载量、较高的存活率以及上调的STAT92E表达。这些数据表明wsv187通过激活宿主JAK/STAT信号通路在控制病毒复制中发挥作用。
