Al-Jiffri Osama H, Al-Sharif Fadwa M, Al-Jiffri Essam H, Uversky Vladimir N
Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University , Jeddah, Saudi Arabia.
Faculty of Science, Department of Biological Science, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Molecular Medicine and USF Health Byrd Alzheimer Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA; Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
Intrinsically Disord Proteins. 2016 May 11;4(1):e1171278. doi: 10.1080/21690707.2016.1171278. eCollection 2016.
Type 2 diabetes mellitus (T2DM) is a chronic and progressive disease that is strongly associated with various complications including cardiovascular diseases and related mortality. The present study aimed to analyze the abundance and functionality of intrinsically disordered regions in several biomarkers of insulin resistance, adiponectin, and endothelial dysfunction found in the T2DM patients. In fact, in comparison to controls, obese T2DM patients are known to have significantly higher levels of inter-cellular adhesion molecule (iCAM-1), vascular cell adhesion molecule (vCAM-1), and E-selectin, whereas their adiponectin levels are relatively low. Bioinformatics analysis revealed that these selected biomarkers (iCAM-1, vCAM-1, E-selectin, and adiponectin) are characterized by the noticeable levels of intrinsic disorder propensity and high binding promiscuity, which are important features expected for proteins serving as biomarkers. Within the limit of studied groups, there is an association between insulin resistance and both hypoadiponectinemia and endothelial dysfunction.
2型糖尿病（T2DM）是一种慢性进行性疾病，与包括心血管疾病及相关死亡率在内的各种并发症密切相关。本研究旨在分析T2DM患者体内胰岛素抵抗、脂联素和内皮功能障碍等几种生物标志物中内在无序区域的丰度和功能。事实上，与对照组相比，已知肥胖的T2DM患者细胞间黏附分子（iCAM-1）、血管细胞黏附分子（vCAM-1）和E-选择素水平显著更高，而其脂联素水平相对较低。生物信息学分析表明，这些选定的生物标志物（iCAM-1、vCAM-1、E-选择素和脂联素）具有显著的内在无序倾向水平和高结合混杂性，这是作为生物标志物的蛋白质所具有的重要特征。在所研究的组范围内，胰岛素抵抗与低脂联素血症和内皮功能障碍之间存在关联。
