活大肠杆菌中的DNA凝聚为转译提供了证据。

DNA condensation in live E. coli provides evidence for transertion.

作者信息

Gorle Anil K, Bottomley Amy L, Harry Elizabeth J, Collins J Grant, Keene F Richard, Woodward Clifford E

机构信息

School of Physical, Environmental and Mathematical Sciences, University of New South Wales, Australian Defence Force Academy, Canberra, ACT 2600, Australia.

The ithree institute, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia.

出版信息

Mol Biosyst. 2017 Mar 28;13(4):677-680. doi: 10.1039/c6mb00753h.

DOI:10.1039/c6mb00753h

PMID:28232991

Abstract

Condensation studies of chromosomal DNA in E. coli with a tetranuclear ruthenium complex are carried out and images obtained with wide-field fluorescence microscopy. Remarkably different condensate morphologies resulted, depending upon the treatment protocol. The occurrence of condensed nucleoid spirals in live bacteria provides evidence for the transertion hypothesis.

摘要

利用一种四核钌配合物对大肠杆菌中的染色体DNA进行凝聚研究，并通过宽场荧光显微镜获得图像。根据处理方案的不同，产生了显著不同的凝聚物形态。活细菌中凝聚类核螺旋的出现为转运假说提供了证据。