Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
Nat Commun. 2023 Mar 2;14(1):1178. doi: 10.1038/s41467-023-36762-z.
It has been proposed that bacterial membrane proteins may be synthesized and inserted into the membrane by a process known as transertion, which involves membrane association of their encoding genes, followed by coupled transcription, translation and membrane insertion. Here, we provide evidence supporting that the pathogen Vibrio parahaemolyticus uses transertion to assemble its type III secretion system (T3SS2), to inject virulence factors into host cells. We propose a two-step transertion process where the membrane-bound co-component receptor (VtrA/VtrC) is first activated by bile acids, leading to membrane association and expression of its target gene, vtrB, located in the T3SS2 pathogenicity island. VtrB, the transmembrane transcriptional activator of T3SS2, then induces the localized expression and membrane assembly of the T3SS2 structural components and its effectors. We hypothesize that the proposed transertion process may be used by other enteric bacteria for efficient assembly of membrane-bound molecular complexes in response to extracellular signals.
有人提出，细菌膜蛋白可能通过一种称为转位(transertion)的过程被合成并插入到膜中，该过程涉及它们编码基因的膜结合，随后是转录、翻译和膜插入的偶联。在这里，我们提供了支持病原体副溶血性弧菌(Vibrio parahaemolyticus) 使用转位(transertion) 来组装其三型分泌系统 (T3SS2)，将毒力因子注入宿主细胞的证据。我们提出了一个两步转位(transertion) 过程，其中膜结合的共组分受体(VtrA/VtrC) 首先被胆汁酸激活，导致其靶基因 vtrB 的膜结合和表达，vtrB 位于 T3SS2 致病性岛。T3SS2 的跨膜转录激活子 VtrB 然后诱导 T3SS2 结构成分及其效应物的局部表达和膜组装。我们假设所提出的转位(transertion) 过程可能被其他肠道细菌用于响应细胞外信号有效地组装膜结合的分子复合物。
