Meymandi Manzumeh-Shamsi, Sepehri Gholamreza, Abdolsamadi Mona, Shaabani Mohammad, Heravi Gioia, Yazdanpanah Omid, Aghtaei Mohammadmehdi-Moeini
Kerman Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran.
Physiology and Pharmacology Department, Kerman Medical School, Kerman University of Medical Sciences, Kerman, Iran.
Inflammopharmacology. 2017 Apr;25(2):237-246. doi: 10.1007/s10787-017-0325-4. Epub 2017 Feb 23.
This study was performed to evaluate the effect of pregabalin co-administration with vitamin E in Partial Sciatic Nerve Ligation (PSNL)-induced neuropathic pain in rats.
Male Wistar rats were randomly allocated as control, sham, and PSNL groups (n = 8). PSNL was induced by tight ligation of the sciatic nerve with a copper wire. On day 14th, the PSNL and sham operated rats received either pregabalin (1, 3, and 30 mg/kg), vitamin E (100 and 200 mg/kg), or their combination intraperitoneally. An antinociceptive effect was evaluated as latency times and Maximum possible Effect Percent (%MPE) using tail-flick test. Locomotor activity was evaluated by open-field test before PSNL surgery and then twice at the 14th days (before and after drug injection). Ligated nerves were removed on the 28th days after surgery for histological examinations.
The time course of latency times and %MPE showed significant decrease in PSNL but not in sham and control groups. Pregabalin (3 and 30 mg/kg) and vitamin E (100 and 200 mg/kg) caused significant increases in latency time in PSNL (but not sham) group compared to control group. Vitamin E 200 mg/kg increased significantly %MPE in PSNL group compared to sham group. In addition, the %MPE following combination treatment of pregabalin (30 mg/kg) and vitamin E (100 mg/kg) was significantly higher than both vitamin E and control group. Also combination of pregabalin with 100 mg/kg of vitamin E reversed Wallerian degeneration of sciatic nerve and the inflammatory responses to almost similar to sham group. Pregabalin and vitamin E did not affect locomotor activity.
Our results showed antinociceptive effects of both vitamin E and pregabalin alone or in combination in PSNL rats and also neuroprotective properties without affecting locomotor activity.
本研究旨在评估加巴喷丁与维生素E联合应用对大鼠坐骨神经部分结扎（PSNL）诱导的神经性疼痛的影响。
将雄性Wistar大鼠随机分为对照组、假手术组和PSNL组（n = 8）。通过用铜丝紧密结扎坐骨神经诱导PSNL。在第14天，PSNL组和假手术组大鼠腹腔注射加巴喷丁（1、3和30 mg/kg）、维生素E（100和200 mg/kg）或它们的组合。使用甩尾试验将镇痛效果评估为潜伏时间和最大可能效应百分比（%MPE）。在PSNL手术前通过旷场试验评估运动活动，然后在第14天（药物注射前后）评估两次。在手术后第28天取出结扎的神经进行组织学检查。
潜伏时间和%MPE的时间进程显示PSNL组显著降低，而假手术组和对照组未降低。与对照组相比，加巴喷丁（3和30 mg/kg）和维生素E（100和200 mg/kg）使PSNL组（而非假手术组）的潜伏时间显著增加。与假手术组相比，维生素E 200 mg/kg使PSNL组的%MPE显著增加。此外，加巴喷丁（30 mg/kg）和维生素E（100 mg/kg）联合治疗后的%MPE显著高于维生素E组和对照组。加巴喷丁与100 mg/kg维生素E的组合还逆转了坐骨神经的华勒氏变性和炎症反应，几乎与假手术组相似。加巴喷丁和维生素E不影响运动活动。
我们的结果表明，维生素E和加巴喷丁单独或联合应用对PSNL大鼠具有镇痛作用，并且具有神经保护特性，而不影响运动活动。
