Enkhtaivan Gansukh, Muthuraman Pandurangan, Kim Doo Hwan
Department of Bioresources and Food Science, Konkuk University, Seoul, 143-701, South Korea.
J Mol Recognit. 2017 Aug;30(8). doi: 10.1002/jmr.2616. Epub 2017 Feb 24.
Influenza virus has had a high rate of antigenic shift and drift that causes significant morbidity and mortality in humans and animals. The lack of excellent pharmacological treatment underlines the importance of the development of the novel antiviral drugs. We investigated the anti-influenza A and B viruses of 2,4-dichlorophenoxyacetic acid (2,4-D), which is the synthetic analog to auxin and is used as a popular herbicide in the agricultural practices. 2,4-D was evaluated using a cytopathic effect reduction method; assay results showed that 2,4-D possessed strong anti-influenza A and B viruses inhibiting the formation of a visible cytopathic effect. Influenza viral RNA expression was performed by quantitative real-time polymerase chain reaction. 2,4-D also inhibited virus replication in the early stage of influenza virus infection without direct interaction with virus particles. Additionally, 2,4-D significantly inhibited various factors occur during influenza virus infection as the acidic vesicular formation and reactive oxygen species production. Moreover, 2,4-D represented no cytotoxicity in normal kidney cell. Therefore, these findings provide an understanding of the mechanism and efficient use of 2,4-D in pharmacological applications against influenza virus infection.
流感病毒具有较高的抗原转变和漂移率，可导致人类和动物出现严重的发病和死亡情况。缺乏有效的药物治疗凸显了开发新型抗病毒药物的重要性。我们研究了2,4-二氯苯氧乙酸（2,4-D）对甲型和乙型流感病毒的作用，2,4-D是生长素的合成类似物，在农业实践中用作一种常用除草剂。采用细胞病变效应减少法对2,4-D进行评估；试验结果表明，2,4-D具有强大的抗甲型和乙型流感病毒能力，可抑制明显细胞病变效应的形成。通过定量实时聚合酶链反应进行流感病毒RNA表达分析。2,4-D在流感病毒感染早期也能抑制病毒复制，且不与病毒颗粒直接相互作用。此外，2,4-D显著抑制流感病毒感染过程中出现的各种因素，如酸性囊泡形成和活性氧产生。此外，2,4-D对正常肾细胞无细胞毒性。因此，这些发现有助于理解2,4-D在抗流感病毒感染药理应用中的作用机制和有效利用。
