Department of Chemistry, University of Texas at Austin , Austin, Texas 78712, United States.
Division of Chemical Biology and Medicinal Chemistry, College of Pharmacy, University of Texas at Austin , Austin, Texas 78712, United States.
Org Lett. 2017 Mar 17;19(6):1426-1429. doi: 10.1021/acs.orglett.7b00355. Epub 2017 Feb 24.
Genome scanning of Streptomyces kaniharaensis, the producer of formycin A, reveals two sets of purA, purB, purC, and purH genes. The Pur enzymes catalyze pyrimidine assembly of purine nucleobases. To test whether enzymes encoded by the second set of pur genes catalyze analogous transformations in formycin biosynthesis, formycin B 5'-phosphate was synthesized and shown to be converted by ForA and ForB to formycin A 5'-phosphate. These results support that For enzymes are responsible for formycin formation.
链霉菌属坎哈拉ensis 是合成(formycin A)的产生菌,对其基因组扫描揭示了两套 purA、purB、purC 和 purH 基因。Pur 酶催化嘧啶核苷酸碱基的嘌呤合成。为了测试第二套 pur 基因编码的酶是否在(formycin A)生物合成中催化类似的转化,合成了(formycin B 5'-phosphate)并证实它可被 ForA 和 ForB 转化为(formycin A 5'-phosphate)。这些结果支持 For 酶负责(formycin A)的形成。