Department of Chemical Engineering, Hanyang University , Ansan, Gyeonggi-do 426-791, Republic of Korea.
ACS Appl Mater Interfaces. 2017 Mar 15;9(10):8581-8588. doi: 10.1021/acsami.6b16783. Epub 2017 Mar 3.
Well-designed scaffolds provide appropriate niches that can effectively recruit host cells and induce differentiation of recruited cells into the desired cell types, facilitating in situ tissue regeneration. Here we report a tissue-specific extracellular matrix (ECM) hydrogel composed of adipose-derived soluble ECM (sECM) and methylcellulose (MC) as a cell-free scaffold system for adipose tissue regeneration. The sECM-MC hydrogels showed a thermosensitive sol-gel phase transition and rapidly formed a soft hydrogel with a stiffness of 3.8 kPa at body temperature. An in vivo study showed that the sECM-MC hydrogel facilitated the infiltration of host cell populations, particularly adipose-derived stem cells (ASCs) and adipose tissue macrophages (ATMs) that directly contribute to the adipose tissue regeneration. Moreover, the hydrogel significantly enhanced host-derived adipogenesis and angiogenesis without exogenous cells or bioactive molecules. Our results indicate that the sECM-MC hydrogels provide mechanical and biochemical cues for host-derived adipose regeneration. Overall, the sECM-MC hydrogels are a highly promising cell-free therapeutic approach for in situ adipose tissue regeneration.
设计良好的支架为宿主细胞提供了合适的微环境，能有效地招募宿主细胞，并诱导募集细胞向所需的细胞类型分化，从而促进原位组织再生。在这里，我们报告了一种组织特异性细胞外基质（ECM）水凝胶，它由脂肪来源的可溶性 ECM（sECM）和甲基纤维素（MC）组成，是一种无细胞支架系统，用于脂肪组织再生。sECM-MC 水凝胶具有温敏溶胶-凝胶相转变特性，在体温下迅速形成柔软的水凝胶，其硬度为 3.8 kPa。体内研究表明，sECM-MC 水凝胶促进了宿主细胞群体的浸润，特别是脂肪来源的干细胞（ASCs）和脂肪组织巨噬细胞（ATMs），它们直接促进脂肪组织再生。此外，该水凝胶在没有外源性细胞或生物活性分子的情况下，显著增强了宿主来源的成脂和成血管作用。我们的结果表明，sECM-MC 水凝胶为宿主来源的脂肪再生提供了机械和生化线索。总的来说，sECM-MC 水凝胶是一种很有前途的无细胞治疗方法，可用于原位脂肪组织再生。
