Díaz-Huerta Hugo, García-Salazar Eduardo, Ramírez-Magaña Xóchitl, Martínez-Herrera Erick, Pinto-Almazán Rodolfo, Betancourt-Cisneros Paola, Duarte-Escalante Esperanza, Reyes-Montes María Del Rocío, Hernández-Castro Rigberto, Frías-De-León María Guadalupe
Programa de Maestría en Ciencias de la Salud, Escuela Superior de Medicina, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Casco de Santo Tomas, Alcaldía Miguel Hidalgo, Ciudad de México CP 11340, Mexico.
Unidad de Calidad y Riesgo Biológico, Hospital Regional de Alta Especialidad de Ixtapaluca, Servicios de Salud del Instituto Mexicano de Seguro Social para el Bienestar (IMSS-BIENESTAR), Carretera Federal México-Puebla Km 34.5, Estado de México CP 56530, Mexico.
J Fungi (Basel). 2025 May 2;11(5):354. doi: 10.3390/jof11050354.
Vulvovaginal candidiasis (VVC) is an opportunistic mycosis that affects women of reproductive age. The most frequent etiological agent is . The development of VVC depends on factors related to the host and the fungus. Among the factors related to spp. are virulence factors, but genotype may also be involved. The objective of this study was to evaluate the ABC genotypes and extracellular hydrolytic enzyme production in isolates obtained from Mexican women with vulvovaginitis to determine if there is a correlation between these characteristics that allows the fungus to invade and cause damage to the host. Forty-three yeast isolates were obtained from vaginal exudates from women with symptoms of infection. The isolates were identified by germ tube tests and by Cand PCR. The ABC genotype of the isolates identified as was determined through the isolates' DNA amplification using the oligonucleotides CA-INT-R and CA-INT-L. The activity of esterase, phospholipase, proteinase, and hemolysin was evaluated in specific culture media. The correlation between extracellular enzyme production and genotype was analyzed using a two-way ANOVA and the Sidak comparison test. A total of 57.5% of the yeast isolates were identified as . The genotypes identified were A (82.6%) and B (17.4%). The activity of esterase, phospholipase, proteinase, and hemolysin was very strong. No statistically significant difference was found between enzyme production and genotypes. In conclusion, genotype A predominates among vaginal isolates. The production of extracellular hydrolytic enzymes was widely expressed in vaginal isolates, but no correlation with genotype was found.
外阴阴道念珠菌病（VVC）是一种影响育龄女性的机会性真菌病。最常见的病原体是 。VVC的发生取决于与宿主和真菌相关的因素。在与 属相关的因素中，毒力因子是其中之一，但基因型也可能起作用。本研究的目的是评估从患有阴道炎的墨西哥女性中分离出的 菌株的ABC基因型和细胞外水解酶的产生情况，以确定这些特征之间是否存在相关性，从而使真菌能够侵入并对宿主造成损害。从有感染症状的女性阴道分泌物中获得了43株酵母菌株。通过芽管试验和念珠菌PCR对菌株进行鉴定。使用寡核苷酸CA-INT-R和CA-INT-L对鉴定为 的菌株进行DNA扩增，从而确定其ABC基因型。在特定培养基中评估酯酶、磷脂酶、蛋白酶和溶血素的活性。使用双向方差分析和Sidak比较检验分析细胞外酶产生与基因型之间的相关性。总共57.5%的酵母菌株被鉴定为 。鉴定出的基因型为A（82.6%）和B（17.4%）。酯酶、磷脂酶、蛋白酶和溶血素的活性非常强。在酶产生和基因型之间未发现统计学上的显著差异。总之，基因型A在 阴道分离株中占主导地位。细胞外水解酶的产生在 阴道分离株中广泛表达，但未发现与基因型相关。
