Seigneurin J M, Desgranges C
Laboratoire de Virologie, Centre Hospitalier Universitaire, Grenoble.
Ann Med Interne (Paris). 1987;138(5):343-6.
Specific human monoclonal anti-CMV antibodies have been isolated and characterized. The first patient had a chronic T cell lymphocytic leukaemia and a subclinical CMV infection developed at the same time as a monoclonal peak of kappa IgG3. The purified F (ab')2 fragment of the IgG3 had an intense anti-CMV activity. A second monoclonal antibody, also a kappa IgG3, was isolated in a non-immunodepressed patient with a primary CMV infection (chronic pyrexia and hepatitis). The immunotransfer showed that the anti-CMV IgM and IgG of the patient's serum reacted particularly with the p51 protein of virus capsid. The monoclonal IgG3 was specific for the same p51. The third monoclonal anti-CMV Ig studied was synthesized in vitro by the B lymphocytes of a renal transplant patient immortalized by the Epstein-Barr virus. This kappa IgM produced continuously reacted strongly with the nucleus of the cells infected by the CMV. Human monoclonal anti-CMV antibodies could be used for the early detection of viral antigens by immunofluorescence and might also be used to treat severe cases of CMV infection.
特异性人抗巨细胞病毒单克隆抗体已被分离和鉴定。首例患者患有慢性T细胞淋巴细胞白血病，在κ IgG3单克隆峰出现的同时发生了亚临床巨细胞病毒感染。IgG3的纯化F(ab')2片段具有强烈的抗巨细胞病毒活性。第二种单克隆抗体也是κ IgG3，是在一名原发性巨细胞病毒感染（慢性发热和肝炎）的非免疫抑制患者中分离得到的。免疫印迹显示，患者血清中的抗巨细胞病毒IgM和IgG特别与病毒衣壳的p51蛋白发生反应。单克隆IgG3对相同的p51具有特异性。研究的第三种抗巨细胞病毒单克隆Ig是由经爱泼斯坦-巴尔病毒永生化的肾移植患者的B淋巴细胞在体外合成的。这种持续产生的κ IgM与巨细胞病毒感染的细胞的细胞核强烈反应。人抗巨细胞病毒单克隆抗体可用于通过免疫荧光早期检测病毒抗原，也可能用于治疗严重的巨细胞病毒感染病例。
