Sauvat A, Zhou H, Leduc M, Gomes-da-Silva L C, Bezu L, Müller K, Forveille S, Liu P, Zhao L, Kroemer G, Kepp O
Metabolomics and Cell Biology Platforms, Gustave Roussy Comprehensive Cancer Institute, Villejuif, France; Equipe 11 labellisée Ligue contre le Cancer, Centre de Recherche des Cordeliers, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Université Pierre et Marie Curie, Paris, France.
Metabolomics and Cell Biology Platforms, Gustave Roussy Comprehensive Cancer Institute, Villejuif, France; Equipe 11 labellisée Ligue contre le Cancer, Centre de Recherche des Cordeliers, Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Université Pierre et Marie Curie, Paris, France; University of Paris Sud XI, Kremlin Bicêtre, France.
Methods Enzymol. 2017;588:219-230. doi: 10.1016/bs.mie.2016.09.082. Epub 2016 Nov 23.
Mitophagy is a peculiar form of organelle-specific autophagy that targets mitochondria. This process ensures cellular homeostasis, as it fosters the disposal of aged and damaged mitochondria that otherwise would be prone to produce reactive oxygen species and hence endanger genomic stability. Similarly, autophagic clearance of depolarized mitochondria plays a fundamental role in organismal homeostasis as exemplified by the link between Parkinson disease and impaired function of the mitophagy-mediating proteins PINK1 and Parkin. Here, we detail an image-based approach for the quantification of mitochondrial Parkin translocation, which is mechanistically important for the initiation of mitophagy.
线粒体自噬是一种针对线粒体的特殊形式的细胞器特异性自噬。这一过程确保细胞内稳态，因为它促进了老化和受损线粒体的清除，否则这些线粒体容易产生活性氧，从而危及基因组稳定性。同样，去极化线粒体的自噬清除在机体稳态中发挥着重要作用，帕金森病与线粒体自噬介导蛋白PINK1和Parkin功能受损之间的联系就是例证。在此，我们详细介绍一种基于图像的方法来定量线粒体Parkin易位，这在机制上对线粒体自噬的启动很重要。
