Pascottini O Bogado, Hostens Miel, Sys P, Vercauteren P, Opsomer G
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium.
Theriogenology. 2017 Apr 1;92:1-5. doi: 10.1016/j.theriogenology.2017.01.004. Epub 2017 Jan 6.
In this study, we aimed to determine risk factors associated with cytological endometritis (CYTO) diagnosed at artificial insemination (AI) in dairy cows. The CYTO risk factors were evaluated based on 1.625 AI-CYTO samples obtained from 873 Holstein-Friesian cows from in total 18 dairy herds in Flanders (Belgium). The endometrial cytology samples were obtained using the cytotape technique, which consisted of adapting a 1.5 cm piece of paper tape to a standardly loaded AI catheter, covered with a double guard sheet. The polymorphonuclear cells' (PMNs) cut-off point for CYTO at AI was set at ≥ 1%. We constructed multilevel generalized mixed effect models in order to identify the risk factors associated with the presence of CYTO at AI. The CYTO prevalence at AI was 27.8% at the animal level, while the within-herd level prevalence ranged from 10.7 to 39.7%, with an average of 28.1%. Risk factors associated with the occurrence of CYTO were parity ≥2 [odds ratio (OR) = 1.8], days in milk (DIM) at AI ≥ 124 (OR = 0.4), and warm months of the year [July (OR = 2.9), August (OR = 2.3), and September (OR = 1.4)]. In conclusion, the present study supports that multiparous cows and cows that are inseminated in the summer months have a higher risk to suffer from CYTO at insemination, while the risk for CYTO is lower when the insemination is taking place at ≥ 124 DIM.
在本研究中，我们旨在确定与奶牛人工授精（AI）时诊断出的细胞学子宫内膜炎（CYTO）相关的风险因素。基于从比利时弗拉芒地区18个奶牛场的873头荷斯坦 - 弗里生奶牛身上获取的1625份AI - CYTO样本，对CYTO风险因素进行了评估。子宫内膜细胞学样本采用细胞胶带技术获取，该技术是将一条1.5厘米长的纸带适配到标准装载的AI导管上，并覆盖双层防护纸。AI时CYTO的多形核细胞（PMN）截断点设定为≥1%。我们构建了多级广义混合效应模型，以识别与AI时CYTO存在相关的风险因素。AI时CYTO在个体水平的患病率为27.8%，而牛群内患病率在10.7%至39.7%之间，平均为28.1%。与CYTO发生相关的风险因素为胎次≥2[比值比（OR）=1.8]、AI时的泌乳天数（DIM）≥124天（OR = 0.4）以及一年中的温暖月份[7月（OR = 2.9）、8月（OR = 2.3）和9月（OR = 1.4）]。总之，本研究支持经产奶牛以及在夏季进行人工授精的奶牛在人工授精时患CYTO的风险更高，而当在泌乳≥124天时进行人工授精时，CYTO的风险较低。
