Bogado Pascottini O, Hostens M, Opsomer G
Faculty of Veterinary Medicine, Department of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, Belgium.
Reprod Domest Anim. 2018 Apr;53(2):559-561. doi: 10.1111/rda.13110. Epub 2017 Nov 14.
This study aimed to (i) assess the prevalence of cytological endometritis (CYTO) diagnosed at artificial insemination (AI); (ii) evaluate the effect of CYTO on the pregnancy outcome of the same AI sample; and (iii) determine the risk factors associated with CYTO diagnosed at AI in repeat breeder (RB) dairy cows. We analysed the productive and reproductive performances of 146 RB Holstein-Friesian cows. To obtain a CYTO sample at AI, we used the cytotape technique. Generalized mixed effect models were computed to find the risk factors associated with the pregnancy and CYTO outcome. Based on ≥1% PMN cut-off point, the CYTO prevalence at AI in RB cows was 25.3%. The overall pregnancy at AI was 44.2%. The conception rate in CYTO-positive (n = 37) RB cows was 29.7% versus 49.5% for CYTO-negative (n = 109) cows. A RB cow diagnosed CYTO positive at AI had 0.47 [odds ratio (OR)] odds to become pregnant in comparison with a CYTO-negative cow. Cows that produced more milk than their counterparts in this study had increased odds (OR = 1.01) to be CYTO positive at AI. A novel risk factor positively associated with CYTO diagnosed at AI in RB cows was the level of daily milk urea (OR = 1.11). To conclude, CYTO at the moment of AI had a significantly negative effect on the pregnancy outcome in RB dairy cows. However, as only one of fourth of RB cows is affected with CYTO at AI, it may not be considered a key element associated with the RB syndrome.
(i)评估人工授精（AI）时诊断出的细胞学子宫内膜炎（CYTO）的患病率；(ii)评估CYTO对同一AI样本妊娠结局的影响；(iii)确定重复配种（RB）奶牛AI时诊断出CYTO的相关风险因素。我们分析了146头RB荷斯坦-弗里生奶牛的生产和繁殖性能。为在AI时获取CYTO样本，我们采用了细胞涂片技术。计算广义混合效应模型以找出与妊娠和CYTO结局相关的风险因素。基于≥1%的中性粒细胞（PMN）临界值，RB奶牛AI时的CYTO患病率为25.3%。AI时的总体妊娠率为44.2%。CYTO阳性（n = 37）的RB奶牛的受孕率为29.7%，而CYTO阴性（n = 109）奶牛的受孕率为49.5%。与CYTO阴性奶牛相比，在AI时被诊断为CYTO阳性的RB奶牛怀孕的几率为0.47[优势比（OR）]。在本研究中，产奶量高于同类奶牛的奶牛在AI时CYTO呈阳性的几率增加（OR = 1.01）。与RB奶牛AI时诊断出CYTO呈正相关的一个新风险因素是每日牛奶尿素水平（OR = 1.11）。总之，AI时的CYTO对RB奶牛的妊娠结局有显著负面影响。然而，由于只有四分之一的RB奶牛在AI时受CYTO影响，它可能不被视为与RB综合征相关的关键因素。
