Sun Lingwei, Guo Yixuan, Fan Yixuan, Nie Haitao, Wang Ruocheng, Wang Feng
Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
Jiangsu Engineering Technology Research Center of Meat Sheep & Goat Industry, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, PR China.
Theriogenology. 2017 Apr 1;92:121-128. doi: 10.1016/j.theriogenology.2017.01.025. Epub 2017 Jan 17.
Nutrition is one of the most important factors affecting the reproductive performance of animals. Changes in the ovine maternal metabolism during pregnancy are critical to fetal development. To understand the differences in ovine metabolic changes that occur during normal pregnancy, pregnant ewes carrying twin fetuses (n = 8) were selected at 35 days of gestation (dG). All ewes received 100% of National Research Council (NRC) requirements of all nutrients and energy during this experiment. At 50, 70, 90, and 110 dG, maternal plasma samples were collected and designated as one of four corresponding time points (T1, T2, T3, and T4, respectively). Maternal plasma samples were analyzed using H nuclear magnetic resonance spectroscopy to compare their metabolomic profiles among time points. We used multivariate pattern recognition to screen for different metabolites in the plasma of the ewes. The body weight and food intake of the ewes were significantly (P < 0.05) different at the four time points, and increased with the passage of pregnancy time. The principal component analysis model results showed that the metabolic states at time points T2 and T3 moved gradually further away from that at T1 and were furthest away from that at T1 at time point T4. Among the different time points, there were thirteen significantly differential metabolites in the maternal plasma (P < 0.05). These metabolites were closely related to amino acid metabolism and lipid metabolism, which might occur at different time points in pregnant ewes. In particular, newly observed changes in 1-methylhistidine and malonate were the first such changes found in maternal plasma. These results demonstrate that the metabolomics approach has value for evaluating metabolism in pregnancy with advancing gestation. In conclusion, during normal pregnancy in Hu sheep, related metabolites play an important role in amino acid and lipid metabolism for meeting the nutritional demands of pregnant ewes.
营养是影响动物繁殖性能的最重要因素之一。孕期绵羊母体代谢的变化对胎儿发育至关重要。为了解正常孕期绵羊代谢变化的差异，在妊娠35天（dG）时选择怀有双胎的妊娠母羊（n = 8）。在本实验期间，所有母羊均接受了美国国家研究委员会（NRC）规定的所有营养素和能量的100%。在妊娠50、70、90和110天，采集母羊血浆样本，并分别指定为四个相应时间点（分别为T1、T2、T3和T4）之一。使用氢核磁共振波谱分析母羊血浆样本，以比较各时间点之间的代谢组学特征。我们使用多变量模式识别来筛选母羊血浆中的不同代谢物。母羊在四个时间点的体重和采食量差异显著（P < 0.05），且随妊娠时间的推移而增加。主成分分析模型结果表明，T2和T3时间点的代谢状态逐渐远离T1时间点，在T4时间点与T1时间点的代谢状态差异最大。在不同时间点之间，母羊血浆中有13种显著差异的代谢物（P < 0.05）。这些代谢物与氨基酸代谢和脂质代谢密切相关，可能在妊娠母羊的不同时间点发生。特别是，新观察到的1-甲基组氨酸和丙二酸的变化是在母羊血浆中首次发现的此类变化。这些结果表明，代谢组学方法对于评估随着妊娠进展的孕期代谢具有价值。总之，在湖羊正常妊娠期间，相关代谢物在氨基酸和脂质代谢中发挥重要作用，以满足妊娠母羊的营养需求。
