Wongsawad Chalobol, Nantarat Nattawadee, Wongsawad Pheravut
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50202, Thailand; Applied Technology for Biodiversity, Institute for Science and Technology, Chiang Mai University, Chiang Mai 50202, Thailand.
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50202, Thailand.
Asian Pac J Trop Med. 2017 Feb;10(2):165-170. doi: 10.1016/j.apjtm.2017.01.016. Epub 2017 Jan 21.
To examine the taxonomic boundaries in the Stellantchasmus populations in different hosts and reveal the cryptic speciation of the Stellantchasmus in Thailand based on both morphological and molecular approaches.
Stellantchasmus falcatus (S. falcatus) s.l. was collected from different hosts throughout Thailand. The worms were examined and measured. The nuclear ITS2 gene and the mitochondrial COI gene were used to investigate the S. falcatus s.l. from Dermogenus pusillus and Liza subviridis.
Stellantchasmus was one of food-borne trematode that widely distribute throughout Thailand. The comparison of S. falcatus s.l. from Dermogenus pusillus and Liza subviridis indicated a genetic divergence of nuDNA with 3.6% and mtDNA with 19.3%, respectively. Morphological characteristics were fairly different in the ratio of body size, length of prepharynx, and ratio of organ size.
Our results provide initial evidence that S. falcatus s.l. from different hosts tend to be a different species based on both molecular and morphological characters. Cryptic species complexes are generally found among parasites that tend to have large populations and/or rapid evolution. The degree of genetic diversity existing would suggest the practice of targeted regimes to design and minimize these lections of anthelmintic resistance in the future.
基于形态学和分子方法，研究不同宿主中华支睾吸虫种群的分类界限，揭示泰国华支睾吸虫的隐秘物种形成。
从泰国各地不同宿主中收集类华支睾吸虫（Stellantchasmus falcatus，简称S. falcatus）。对虫体进行检查和测量。利用核糖体DNA内转录间隔区2（ITS2）基因和线粒体细胞色素氧化酶亚基I（COI）基因研究来自微小革囊星虫（Dermogenus pusillus）和多鳞𬶮（Liza subviridis）的类华支睾吸虫。
华支睾吸虫是泰国广泛分布的食源性吸虫之一。来自微小革囊星虫和多鳞𬶮的类华支睾吸虫的比较表明，核DNA的遗传差异为3.6%，线粒体DNA的遗传差异为19.3%。在虫体大小比例、咽前体长度和器官大小比例方面，形态特征差异较大。
我们的结果提供了初步证据，表明基于分子和形态特征，来自不同宿主的类华支睾吸虫可能是不同的物种。隐秘物种复合体通常存在于具有大量种群和/或快速进化趋势的寄生虫中。现有的遗传多样性程度表明，未来应采取有针对性的措施来设计和尽量减少这些抗驱虫药选择。
