Lu Zhai, Chen Hua, Zheng Xiao-Mei, Chen Man-Ling
Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan, China.
Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan, China.
Asian Pac J Trop Med. 2017 Feb;10(2):195-200. doi: 10.1016/j.apjtm.2017.01.007. Epub 2017 Jan 19.
To study the expression of E6 and E7 mRNA in high-risk human papillomavirus (HPV) HPV-18 and the relationship between the expression of invasive gene and cervical carcinoma.
A total of 119 patients with cervical cancer, cervical erosion and cervical HPV infection who were diagnosed in our hospital were selected and randomly divided into two groups: cervical cancer group (n = 58) and non-cancerous group (n = 61). Another 60 patients with uterine leiomyoma were selected as normal control group. Detection of HPV18 E6, E7 mRNA expression and invasion, migration, proliferation inhibition genes, epithelial mesenchymal transition genes and proliferation related protein content.
The relative expression of E6 and E7 HPV-18 in cervical cancer group was significant higher than that in non-cancerous group and control group (mRNA) (P < 0.05). The content of TRAF6 and c-FLIP in invasive cervical cancer group was significantly higher than that in non-cancerous group and control group (P < 0.05). The mRNA content of CD44v6 and MMP-9 in cervical cancer group was significantly higher than that in non-cancerous group and control group (P < 0.05). The content of DEC-1, IKK16, MBP-1 in cervical cancer group was significant lower than that in non-cancerous group and control group (P < 0.05). The mRNA content of beta -catenin and Vimentin in cervical cancer group was significantly lower than that in non cancerous group and control group (P < 0.05). The proliferation related protein E2F1 of cervical cancer group was significantly lower than that of non-cancerous group and control group, Bmi-1 content was significantly higher than non-cancerous group and control group (P < 0.05).
The expression of the detection of cervical cancer in high-risk human papilloma virus HPV-18 E6 and E7 mRNA, and the invasion, migration, proliferation inhibition gene, epithelial mesenchymal transition and proliferation related gene protein content, HPV expression rate of mRNA increased with the development of cervical cancer, the expression is also enhanced. The expression has a certain correlation between the level and development of cervical cancer. Through the above indicators, the development of cervical cancer monitoring and treatment to provide important clinical guidance.
研究高危型人乳头瘤病毒（HPV）HPV - 18中E6和E7 mRNA的表达及其与侵袭基因表达和宫颈癌的关系。
选取我院确诊的119例宫颈癌、宫颈糜烂及宫颈HPV感染患者，随机分为两组：宫颈癌组（n = 58）和非癌组（n = 61）。另选取60例子宫肌瘤患者作为正常对照组。检测HPV18 E6、E7 mRNA表达及侵袭、迁移、增殖抑制基因、上皮间质转化基因和增殖相关蛋白含量。
宫颈癌组HPV - 18 E6和E7的相对表达量显著高于非癌组和对照组（mRNA）（P < 0.05）。宫颈癌侵袭组TRAF6和c - FLIP的含量显著高于非癌组和对照组（P < 0.05）。宫颈癌组CD44v6和MMP - 9的mRNA含量显著高于非癌组和对照组（P < 0.05）。宫颈癌组DEC - 1、IKK16、MBP - 1的含量显著低于非癌组和对照组（P < 0.05）。宫颈癌组β - 连环蛋白和波形蛋白的mRNA含量显著低于非癌组和对照组（P < 0.05）。宫颈癌组增殖相关蛋白E2F1显著低于非癌组和对照组，Bmi - 1含量显著高于非癌组和对照组（P < 0.05）。
检测高危型人乳头瘤病毒HPV - 18 E6和E7 mRNA在宫颈癌中的表达，以及侵袭、迁移、增殖抑制基因、上皮间质转化及增殖相关基因蛋白含量，HPV mRNA表达率随宫颈癌的发展而升高，表达也增强。其表达水平与宫颈癌的发展有一定相关性。通过上述指标，为宫颈癌的监测和治疗发展提供重要的临床指导。
