Takeda Kazu, Mori Shiro, Kodama Tetsuya
Laboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan; Biomedical Engineering Cancer Research Center, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan.
Laboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan; Biomedical Engineering Cancer Research Center, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, Japan; Department of Oral and Maxillofacial Surgery, Tohoku University Hospital, 1-1 Seiryo, Aoba, Sendai 980-8575, Japan.
J Immunol Methods. 2017 Jun;445:1-9. doi: 10.1016/j.jim.2017.02.008. Epub 2017 Feb 22.
Cancer cells metastasize to lymph nodes, with distant metastasis resulting in poor prognosis. The role of lymph node metastasis (LNM) in the spread of cancer to distant organs remain incompletely characterized. The visualization of flow dynamics in the lymphatic and blood vessels of MXH10/Mo-lpr/lpr mice, which develop systemic swelling of lymph nodes up to 10mm in diameter, has revealed that lymph nodes have the potential to be a direct source of systemic metastasis. However, it is not known whether these fluid dynamics characteristics are universal phenomena present in other strains of laboratory mice. Here we show that the fluid dynamics observed in MXH10/Mo-lpr/lpr mice are the same as those observed in C57BL/6J, BALB/cAJcl and NOD/ShiJic-scidJcl mice. Furthermore, when fluorescent solution was injected into a tumor-bearing lymph node, the flow dynamics observed in the efferent lymphatic vessels and thoracoepigastric vein depended on the type of tumor cell. Our results indicate that fluid dynamics in the lymphatic and blood vessels of MXH10/Mo-lpr/lpr mice are generalized phenomena seen in conventional laboratory mice. We anticipate our results can facilitate studies of the progression of lymphatic metastasis to hematogenous metastasis via lymph nodes and the early diagnosis and treatment of LNM.
癌细胞会转移至淋巴结，远处转移会导致预后不良。淋巴结转移（LNM）在癌症扩散至远处器官过程中的作用仍未完全明确。MXH10/Mo-lpr/lpr小鼠会出现直径达10毫米的全身性淋巴结肿大，对其淋巴管和血管内血流动力学的可视化研究表明，淋巴结有可能是全身性转移的直接来源。然而，尚不清楚这些流体动力学特征是否是其他品系实验小鼠中普遍存在的现象。在此我们表明，在MXH10/Mo-lpr/lpr小鼠中观察到的流体动力学与在C57BL/6J、BALB/cAJcl和NOD/ShiJic-scidJcl小鼠中观察到的相同。此外，当将荧光溶液注入荷瘤淋巴结时，在输出淋巴管和胸腹壁静脉中观察到的血流动力学取决于肿瘤细胞的类型。我们的结果表明，MXH10/Mo-lpr/lpr小鼠淋巴管和血管中的流体动力学是常规实验小鼠中普遍存在的现象。我们预计我们的结果能够促进对通过淋巴结从淋巴转移进展为血行转移以及LNM的早期诊断和治疗的研究。
