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氨对淡水剑水蚤(Eucyclops serrulatus)基因表达水平的影响。

Effect of ammonia on the gene expression levels of the freshwater cyclopoid Eucyclops serrulatus.

作者信息

Di Lorenzo Tiziana, Melita Marco, Cifoni Marco, Galassi Diana M P, Iannucci Alessio, Biricolti Stefano, Gori Massimo, Baratti Mariella

机构信息

Institute of Ecosystem Study (ISE), CNR, Sesto Fiorentino, Firenze, Italy.

Institute of Ecosystem Study (ISE), CNR, Sesto Fiorentino, Firenze, Italy; Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.

出版信息

Environ Toxicol Pharmacol. 2017 Apr;51:138-141. doi: 10.1016/j.etap.2017.02.017. Epub 2017 Feb 21.

DOI:10.1016/j.etap.2017.02.017
PMID:28238699
Abstract

Ammonia pollution is a critical issue in Europe, since more than half of the European freshwater bodies actually fail to meet EU quality standards for this chemical. In this study, the response of stress-related genes to a sublethal ammonia concentration has been investigated in the adults of the freshwater cyclopoid Eucyclops serrulatus. Two short-term exposures (12h and 24h) at 12mg/L NH have been tested. Results indicate that 12mg/L NH causes a significant increase in the expression of some proteins, namely CAT, HSP90 and HSP40, suggesting an activation of the protecting antioxidant system after both 12h and 24h.

摘要

氨污染是欧洲的一个关键问题,因为超过一半的欧洲淡水水体实际上未能达到欧盟对这种化学物质的质量标准。在本研究中,已经在淡水剑水蚤锯缘真剑水蚤的成体中研究了应激相关基因对亚致死氨浓度的反应。测试了在12mg/L NH₃下的两次短期暴露(12小时和24小时)。结果表明,12mg/L NH₃会导致一些蛋白质(即CAT、HSP90和HSP40)的表达显著增加,这表明在12小时和24小时后保护抗氧化系统均被激活。

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