Yamamoto T, Takahashi S, Moriwaki Y, Hada T, Higashino K
Third Department of Internal Medicine, Hyogo College of Medicine, Japan.
Biochim Biophys Acta. 1987 Nov 21;922(2):177-83. doi: 10.1016/0005-2760(87)90152-4.
Apolipoprotein B-containing high-density lipoprotein was detected in the high-density lipoprotein fraction of the concentrated conditioned medium of human hepatoma HuH-7 cells (Apo-B-containing HDLHuH-7). Electrophoretically, the Apo-B-containing HDLHuH-7 migrated more slowly and broadly than beta-lipoprotein on agarose gel. The Apo-B-containing HDLHuH-7 was not precipitated by heparin-Mn2+ treatment. High-performance gel filtration chromatography indicated that the peak of Apo-B-containing HDLHuH-7 was eluted faster than that of plasma LDL and electron microscopic analysis demonstrated that the particles of Apo-B-containing HDLHuH-7 ranged from 140 A to about 450 A, indicating the heterogeneity of Apo-B-containing HDLHuH-7. The protein/lipid ratio of HDLHuH-7 (1.35) is higher than that of plasma HDL3 (1.2). The lipids of HDLHuH-7 are composed of phospholipids (170 micrograms/mg protein), free cholesterol (410 micrograms/mg protein), cholesterol ester (20 micrograms/mg protein) and triacylglycerols (140 micrograms/mg protein). The results described above indicated that Apo-B-containing HDLHuH-7 was a novel lipoprotein discovered for the first time.
在人肝癌HuH-7细胞浓缩条件培养基的高密度脂蛋白组分中检测到含载脂蛋白B的高密度脂蛋白（含Apo-B的HDLHuH-7）。在琼脂糖凝胶上进行电泳时，含Apo-B的HDLHuH-7迁移速度比β-脂蛋白更慢且更宽。含Apo-B的HDLHuH-7不会被肝素-Mn2+处理沉淀。高效凝胶过滤色谱表明，含Apo-B的HDLHuH-7的峰比血浆低密度脂蛋白洗脱得更快，电子显微镜分析表明，含Apo-B的HDLHuH-7的颗粒大小在140埃至约450埃之间，表明含Apo-B的HDLHuH-7具有异质性。HDLHuH-7的蛋白质/脂质比（1.35）高于血浆HDL3（1.2）。HDLHuH-7的脂质由磷脂（170微克/毫克蛋白质）、游离胆固醇（410微克/毫克蛋白质）、胆固醇酯（20微克/毫克蛋白质）和三酰甘油（140微克/毫克蛋白质）组成。上述结果表明，含Apo-B的HDLHuH-7是首次发现的一种新型脂蛋白。
