Stancheva Mona, Dobreva Diana A
Department of Chemistry, Faculty of Pharmacy, Medical University of Varna, 55 Marin Drinov str., Varna, 9000, Bulgaria.
Foods. 2013 Jul 19;2(3):332-337. doi: 10.3390/foods2030332.
The aim of the present study evaluates the fat-soluble vitamins all- retinol (vitamin A), cholecalciferol (vitamin D₃) and α-tocopherol (vitamin E) content in the fresh edible tissue of Bulgarian fish species: marine-grey mullet () and bonito (), and freshwater-rainbow trout () and common carp (). The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction with -hexane. All- retinol, cholecalciferol and α-tocopherol were analyzed simultaneously using RP-HPLC\UV\FL system with analytical column C18 ODS2 Hypersil™. The fat soluble vitamins content (μg per 100 g wet weight) in the fresh edible fish tissue of analyzed fishes are in the ranges: vitamin A from 2.7 ± 0.4 to 37.5 ± 3.4 μg/100 g ww; vitamin D₃ from 1.1 ± 0.1 to 11.4 ± 0.6 μg/100 g ww; vitamin E from 121.4 ± 9.6 to 1274.2 ± 44.1 μg/100 g ww. Three fat-soluble vitamins occur in higher amounts in rainbow trout and grey mullet species. According to recommended daily intake (RDI), they are a good source of cholecalciferol.
本研究旨在评估保加利亚鱼类新鲜可食用组织中脂溶性维生素全反式视黄醇（维生素A）、胆钙化醇（维生素D₃）和α-生育酚（维生素E）的含量：海水鱼灰鲻鱼（）和鲣鱼（），以及淡水鱼虹鳟鱼（）和鲤鱼（）。样品制备程序包括碱性皂化，然后用正己烷进行液液萃取。使用配备C18 ODS2 Hypersil™ 分析柱的RP-HPLC\UV\FL系统同时分析全反式视黄醇、胆钙化醇和α-生育酚。所分析鱼类新鲜可食用鱼组织中脂溶性维生素含量（每100克湿重中的微克数）范围如下：维生素A为2.7±0.4至37.5±3.4微克/100克湿重；维生素D₃为1.1±0.1至11.4±0.6微克/100克湿重；维生素E为121.4±9.6至1274.2±44.1微克/100克湿重。虹鳟鱼和灰鲻鱼中三种脂溶性维生素的含量较高。根据推荐每日摄入量（RDI），它们是胆钙化醇的良好来源。
