Maeda Hayato, Hosomi Ryota, Chiba Utako, Fukunaga Kenji
Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori 036-8561, Japan.
Division of Food Science and Nutrition, Tottori College, Kurayoshi, Tottori 682-8555, Japan.
Foods. 2013 Sep 6;2(3):415-429. doi: 10.3390/foods2030415.
Salmon ovary outer membrane (SOM) is a byproduct of the salmon industry; however, the effective utilization of SOM for food materials and supplements is anticipated as the demand for fish and seafood increases worldwide. The purposes of the present study were to assess the chemical composition of SOM, the characteristics of SOM protein (SOMP), and its effects on serum and fecal biochemical parameters in mice. SOM contained high levels of crude protein (61.9 g/100 g) and crude lipid (18.9 g/100 g). The protein pattern of SOMP was different from those of fish muscle protein and roe; it was abundant in collagen, as calculated from the hydroxyproline content. In addition, SOMP exhibited lower protein digestibility during digestion analyses compared with casein. Male C57BL/6J and KK- mice were fed a casein-based semi-purified diet or a diet with replacement of part of the dietary protein (50%) by SOMP for four weeks. Mice fed the diet containing SOMP showed elevated fecal nitrogen and mucins contents and reduced levels of serum liver injury markers and fecal ammonia. These results show for the first time that chemical composition of SOM, and SOMP, contain a resistant protein fraction and a large amount of collagen. Therefore, SOM is a potential source of marine collagen and functional food material for promoting the health of the liver and colon.
鲑鱼卵巢外膜（SOM）是鲑鱼产业的一种副产品；然而，随着全球对鱼类和海鲜需求的增加，预计SOM在食品原料和补充剂方面的有效利用前景广阔。本研究的目的是评估SOM的化学成分、SOM蛋白（SOMP）的特性及其对小鼠血清和粪便生化参数的影响。SOM含有高水平的粗蛋白（61.9克/100克）和粗脂肪（18.9克/100克）。SOMP的蛋白质模式与鱼肉蛋白和鱼籽的不同；根据羟脯氨酸含量计算，其富含胶原蛋白。此外，在消化分析中，与酪蛋白相比，SOMP的蛋白质消化率较低。雄性C57BL/6J和KK-小鼠分别喂食基于酪蛋白的半纯化日粮或用SOMP替代部分日粮蛋白（50%）的日粮，持续四周。喂食含SOMP日粮的小鼠粪便氮和粘蛋白含量升高，血清肝损伤标志物和粪便氨水平降低。这些结果首次表明，SOM和SOMP的化学成分含有抗性蛋白部分和大量胶原蛋白。因此，SOM是海洋胶原蛋白的潜在来源和促进肝脏和结肠健康的功能性食品原料。
