Leong Dara, Alvarez-Ordóez Avelino, Guillas Floriane, Jordan Kieran
Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
Foods. 2013 Nov 27;2(4):544-553. doi: 10.3390/foods2040544.
In the EU, food is considered safe with regard to if its numbers do not exceed 100 CFU/g throughout the shelf-life of the food. Therefore, it is important to determine if a food supports growth of . Challenge studies to determine the ability of a food to support growth of are essential as predictive modelling often overestimates the growth ability of . The aim of this study was to determine if growth of was supported during the production and distribution of mushrooms. A three-strain mixture of was inoculated onto three independent batches of whole mushrooms, sliced mushrooms, mushroom casing and mushroom substrate at a concentration of about 100-1000 CFU/g. The batches were incubated at potential abuse temperatures, as a worst case scenario, and at intervals during storage numbers, % moisture and pH were determined. The results showed that the sliced and whole mushrooms had the ability to support growth, while mushroom casing allowed survival but did not support growth. Mushroom substrate showed a rich background microflora that grew on selective media and this hindered enumeration of . In the case of this study, Combase predictions were not always accurate, indicating that challenge studies may be a necessary part of growth determination of .
在欧盟，如果某种食品在其整个保质期内的数量不超过100 CFU/g，则被认为是安全的。因此，确定一种食品是否支持[某种微生物名称]的生长很重要。由于预测模型常常高估[某种微生物名称]的生长能力，所以开展挑战研究以确定一种食品支持[某种微生物名称]生长的能力至关重要。本研究的目的是确定在蘑菇的生产和分销过程中[某种微生物名称]是否能生长。将[某种微生物名称]的三菌株混合物以约100 - 1000 CFU/g的浓度接种到三批独立的完整蘑菇、切片蘑菇、蘑菇覆土和蘑菇培养料上。作为最坏情况，将这些批次在潜在的滥用温度下培养，并在储存期间定期测定[某种微生物名称]数量、水分百分比和pH值。结果表明，切片蘑菇和完整蘑菇有支持[某种微生物名称]生长的能力，而蘑菇覆土能让[某种微生物名称]存活但不支持其生长。蘑菇培养料显示出丰富的背景微生物群落，它们在[某种微生物名称]选择性培养基上生长，这妨碍了[某种微生物名称]的计数。在本研究中，Combase预测并不总是准确的，这表明挑战研究可能是确定[某种微生物名称]生长情况的必要组成部分。
