Stanilov Noyko S, Miteva Lyuba, Cirovski Geo, Stanilova Spaska A
Faculty of Medicine, Trakia University, Stara Zagora, Bulgaria.
Hospital "Trakia", Stara Zagora, Bulgaria.
Contemp Oncol (Pozn). 2016;20(6):458-462. doi: 10.5114/wo.2016.65605. Epub 2017 Jan 12.
The ability of immune cells in peripheral blood to produce certain cytokines affects tumour-elicited inflammation. The aim of this study was to investigate the gene expression of interleukin 12A (IL-12A), IL-12B, IL-23A, IL-10, IL-6, transforming growth factor β (TGF-β), HDAC3, and iNOS in peripheral blood mononuclear cells (PBMC) from colorectal cancer (CRC) patients.
The venous blood for PBMC isolation was collected preoperatively and 10 days after surgery, from CRC patients. After isolation of total RNA and synthesis of cDNA, quantitative real-time PCR assays were performed.
Our results demonstrated that among investigated cytokine genes IL-10 and TGF-β were significantly upregulated in patients with CRC compared to the control group, while the expression of IL-23 mRNA was significantly decreased in CRC patients. We observed significantly increased mRNA levels in CRC patients' PBMC before surgery for IL-10 and TGF-β compared to both postoperative and control groups. We also found a significant upregulation of iNOS in early compared to advanced CRC.
Based on the results we can assume that PBMC gene expression programming in CRC patients drives local differentiation of Th cells towards Treg instead of the Th1 anti-tumour subpopulation.
外周血免疫细胞产生特定细胞因子的能力会影响肿瘤引发的炎症。本研究旨在调查结直肠癌（CRC）患者外周血单个核细胞（PBMC）中白细胞介素12A（IL-12A）、IL-12B、IL-23A、IL-10、IL-6、转化生长因子β（TGF-β）、组蛋白去乙酰化酶3（HDAC3）和诱导型一氧化氮合酶（iNOS）的基因表达。
从CRC患者术前及术后10天采集用于分离PBMC的静脉血。分离总RNA并合成cDNA后，进行定量实时PCR检测。
我们的结果表明，在研究的细胞因子基因中，与对照组相比，CRC患者中IL-10和TGF-β显著上调，而CRC患者中IL-23 mRNA的表达显著降低。我们观察到，与术后组和对照组相比，CRC患者术前PBMC中IL-10和TGF-β的mRNA水平显著升高。我们还发现，与晚期CRC相比，早期CRC中iNOS显著上调。
基于这些结果，我们可以推测CRC患者PBMC的基因表达编程促使Th细胞向Treg而非Th1抗肿瘤亚群进行局部分化。
