Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN, UMR CNRS 8520), Avenue Poincaré, BP 60069, 59652 Villeneuve d'Ascq, France.
Institut des Biomolécules Max Mousseron (IBMM), UMR 5247, Université de Montpellier, CNRS, ENSCM, Place Eugène Bataillon, 34095 Montpellier cedex 5, France.
Analyst. 2017 Mar 13;142(6):969-978. doi: 10.1039/c6an02588a.
In this paper, we report an original method to immobilize a model peptide on silicon nanowires (SiNWs) via a photolinker attached to the SiNWs' surface. The silicon nanowires were fabricated by a metal assisted chemical etching (MACE) method. Then, direct characterization of the peptide immobilization on SiNWs was performed either by X-ray photoelectron spectroscopy (XPS) or by laser-desorption/ionization mass spectrometry (LDI-MS). XPS allowed us to follow the peptide immobilization and its photorelease by recording the variation of the signal intensities of the different elements present on the SiNW surface. Mass spectrometry was performed without the use of an organic matrix and peptide ions were produced via a photocleavage mechanism. Indeed, thanks to direct photorelease achieved upon laser irradiation, a recorded predictable peak related to the molecular peptide ion has been detected, allowing the identification of the model peptide. Additional MS/MS experiments confirmed the photodissociation site and confirmed the N-terminal immobilization of the peptide on SiNWs.
在本文中，我们报告了一种将模型肽固定在硅纳米线（SiNWs）上的原创方法，该方法通过附着在 SiNWs 表面的光连接子实现。硅纳米线是通过金属辅助化学蚀刻（MACE）方法制备的。然后，通过 X 射线光电子能谱（XPS）或激光解吸/电离质谱（LDI-MS）直接对肽在 SiNWs 上的固定进行了表征。XPS 通过记录 SiNW 表面上存在的不同元素的信号强度的变化，使我们能够跟踪肽的固定及其光释放。质谱分析无需使用有机基质，并且通过光裂解机制产生肽离子。事实上，由于激光照射实现了直接光释放，因此检测到了与分子肽离子相关的可预测峰，从而可以鉴定模型肽。额外的 MS/MS 实验证实了光解离部位，并确认了肽在 SiNWs 上的 N 端固定。
