间充质来源的成纤维样细胞对淋巴细胞针对NK敏感靶细胞的细胞毒活性的影响。
Effect of Fibroblast-Like Cells of Mesenchymal Origin of Cytotoxic Activity of Lymphocytes against NK-Sensitive Target Cells.
作者信息
Lupatov A Yu, Kim Ya S, Bystrykh O A, Vakhrushev I V, Pavlovich S V, Yarygin K N, Sukhikh G T
机构信息
V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2017 Feb;162(4):552-557. doi: 10.1007/s10517-017-3658-5. Epub 2017 Feb 27.
We studied immunosuppressive properties of skin fibroblasts and mesenchymal stromal cells against NK cells. In vitro experiments showed that mesenchymal stromal cells isolated from human umbilical cord and human skin fibroblasts can considerably attenuate cytotoxic activity of NK cells against Jurkat cells sensitive to NK-mediated lysis. NK cells cultured in lymphocyte population exhibited higher cytotoxic activity than isolated NK cells. Mesenchymal stromal cells or fibroblasts added 1:1 to lymphocyte culture almost completely suppressed NK cell cytotoxicity. This suggests that fibroblast-like cells can suppress not only isolated NK cells, but also NK cells in natural cell microenvironment.
我们研究了皮肤成纤维细胞和间充质基质细胞对自然杀伤（NK）细胞的免疫抑制特性。体外实验表明，从人脐带分离的间充质基质细胞和人皮肤成纤维细胞可显著减弱NK细胞对NK介导裂解敏感的Jurkat细胞的细胞毒活性。在淋巴细胞群体中培养的NK细胞比分离的NK细胞表现出更高的细胞毒活性。以1:1比例添加到淋巴细胞培养物中的间充质基质细胞或成纤维细胞几乎完全抑制了NK细胞的细胞毒性。这表明成纤维样细胞不仅可以抑制分离的NK细胞，还可以抑制天然细胞微环境中的NK细胞。
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