Lee J S, Pathak S, Hopwood V, Tomasovic B, Mullins T D, Baker F L, Spitzer G, Neidhart J A
Department of Medical Oncology, University of Texas M. D. Anderson Hospital and Tumor Institute at Houston 77030.
Cancer Res. 1987 Dec 1;47(23):6349-52.
By using the newly developed adhesive tumor cell culture system, we analyzed the chromosomal constitutions of primary lung tumor and nonmalignant normal lung tissue from 10 previously untreated patients with non-small cell lung cancer. Chromosomal analyses were successfully carried out in banded chromosome preparations from 10 tumor and 8 normal lung tissue samples. All analyzed tumor and normal lung tissue samples had a predominantly normal diploid chromosome number. However, there was at least one structural or numerical alteration in every tumor and lung tissue sample analyzed. Chromosomes 1, 3, 4, 6, 7, 8, 9, 12, 15, and 20 were more often involved in rearrangement. The most consistent finding was trisomy 7; 4 patients had trisomy 7 in both tumor and normal lung tissue, and another 2 had this anomaly in tumor tissue only. Of the 4 patients without trisomy 7, 2 had a homogeneously staining region in the short arm of chromosome 7 in tumor tissue. Phytohemagglutinin-stimulated peripheral blood lymphocytes from 7 patients, including 5 patients with trisomy 7 in tumor tissue, did not show trisomy 7. These cytogenetic data suggest that chromosome 7 may be associated with lung cancer development and that trisomy 7 may be the hallmark of premalignant changes, at least in a subgroup of patients with non-small cell lung cancer.
通过使用新开发的黏附肿瘤细胞培养系统，我们分析了10例未经治疗的非小细胞肺癌患者的原发性肺肿瘤和非恶性正常肺组织的染色体组成。在10个肿瘤和8个正常肺组织样本的显带染色体标本上成功进行了染色体分析。所有分析的肿瘤和正常肺组织样本的染色体数目主要为正常二倍体。然而，在每个分析的肿瘤和肺组织样本中至少存在一种结构或数目改变。1、3、4、6、7、8、9、12、15和20号染色体更常发生重排。最一致的发现是7号染色体三体；4例患者的肿瘤和正常肺组织均有7号染色体三体，另外2例仅肿瘤组织有此异常。在4例无7号染色体三体的患者中，2例肿瘤组织的7号染色体短臂有均匀染色区。7例患者（包括5例肿瘤组织有7号染色体三体的患者）经植物血凝素刺激的外周血淋巴细胞未显示7号染色体三体。这些细胞遗传学数据表明，7号染色体可能与肺癌发生有关，且7号染色体三体可能是癌前改变的标志，至少在一部分非小细胞肺癌患者中是如此。
