Knuutila S, Larramendy M L, Elfving P, el-Rifai W, Miettinen A, Mitelman F
Department of Medical Genetics, University of Helsinki, Finland.
Hum Genet. 1995 Feb;95(2):149-56. doi: 10.1007/BF00209393.
The cellular origin of trisomy 7 in non-neoplastic kidney tissue specimens from 10 patients, seven with malignant tumors and three with non-neoplastic kidney diseases, was studied by the MAC (morphology antibody chromosomes) technique, which allows analysis of cellular morphology/histology, immunophenotype, and chromosomal aneuploidy by conventional cytogenetics, and/or fluorescent in situ hybridization in both interphase and mitotic cells. In primary cultures, trisomy 7 was detected primarily in cytokeratin-positive cells. Among freshly isolated renal cells, the trisomy was mainly observed in proximal tubular cells positive to brush-border antigen, and, to a lesser extent, in distal tubular cells positive to Tamm-Horsfall glycoprotein. The frequency of trisomy 7 in lymphocytes expressing CD3 or CD22 antigens isolated from non-neoplastic and tumor tissues was substantially lower than in the epithelial cells and was not increased compared with that in control lymphocytes from peripheral blood. The results thus demonstrate that the non-neoplastic kidney cells with trisomy 7 are mainly normal epithelial cells, preferentially those of the proximal tubule.
运用MAC（形态学-抗体-染色体）技术，对10例患者非肿瘤性肾组织标本中7号染色体三体的细胞起源进行了研究，其中7例患有恶性肿瘤，3例患有非肿瘤性肾脏疾病。该技术可通过传统细胞遗传学方法以及间期和有丝分裂期细胞的荧光原位杂交技术，分析细胞形态学/组织学、免疫表型和染色体非整倍性。在原代培养中，主要在细胞角蛋白阳性细胞中检测到7号染色体三体。在新鲜分离的肾细胞中，三体主要见于刷状缘抗原阳性的近端肾小管细胞，少数见于Tamm-Horsfall糖蛋白阳性的远端肾小管细胞。从非肿瘤组织和肿瘤组织中分离出的表达CD3或CD22抗原的淋巴细胞中，7号染色体三体的频率显著低于上皮细胞，与外周血对照淋巴细胞相比也未增加。因此，结果表明，具有7号染色体三体的非肿瘤性肾细胞主要是正常上皮细胞，尤其是近端小管的上皮细胞。
