Jiang Shan, Miao Bei, Chen Ying
aDepartment of Anesthesiology, The First People's Hospital of Lianyungang bDepartment of Anatomy and Physiology Lianyungang Branch of Traditional Chinese Medicine, Jiangsu Union Technical Institute, Lianyungang cDepartment of Gastroenterology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Neuroreport. 2017 May 3;28(7):386-390. doi: 10.1097/WNR.0000000000000760.
Postoperative cognitive dysfunction is a frequent complication with surgery and anesthesia, and the underlying mechanism is unclear. Our aim was to investigate the effect of different durations of isoflurane anesthesia on spatial recognition memory and activation of JNK1/2 in the hippocampus of mice. In the present study, adult male mice were anesthetized with isoflurane for different durations (1.5% isoflurane for 1, 2, and 4 h). Spatial recognition memory was determined using spontaneous alternation and two-trial recognition memory in Y-maze at 24 h after anesthesia. The activation of JNK1/2 in the hippocampus was tested using western blot. Mice treated with isoflurane for 4 h showed significantly decreased spontaneous alternations and decreased exploration parameters compared with the no anesthesia group, but this was not observed in mice treated with isoflurane for 1 or 2 h. The protein levels of p-JNK1/2 in the hippocampus were significantly increased at 10 min after isoflurane anesthesia for 1, 2, and 4 h compared with no anesthesia. However, only isoflurane anesthesia for 4 h still increased JNK1/2 and p-JNK1/2 levels at 24 h after anesthesia. We concluded that prolonged duration of isoflurane anesthesia maintained the activation of JNK1/2, which led to memory impairment at 24 h after anesthesia.
术后认知功能障碍是手术和麻醉常见的并发症，其潜在机制尚不清楚。我们的目的是研究不同时长的异氟烷麻醉对小鼠空间识别记忆及海马体中JNK1/2激活的影响。在本研究中，成年雄性小鼠用异氟烷麻醉不同时长（1.5%异氟烷，分别麻醉1、2和4小时）。在麻醉后24小时，使用Y迷宫中的自发交替和两次试验识别记忆来测定空间识别记忆。使用蛋白质印迹法检测海马体中JNK1/2的激活情况。与未麻醉组相比，用异氟烷麻醉4小时的小鼠自发交替显著减少，探索参数降低，但用异氟烷麻醉1或2小时的小鼠未观察到这种情况。与未麻醉相比，异氟烷麻醉1、2和4小时后10分钟，海马体中p-JNK1/2的蛋白水平显著升高。然而，仅异氟烷麻醉4小时在麻醉后24小时仍会增加JNK1/2和p-JNK1/2水平。我们得出结论，延长异氟烷麻醉时长会维持JNK1/2的激活，这导致麻醉后24小时出现记忆损伤。
