Department of Biology, Federal University of Lavras, 37.200-000 Lavras, MG, Brazil.
Department of Biology, Federal University of Lavras, 37.200-000 Lavras, MG, Brazil.
Int J Food Microbiol. 2017 May 2;248:39-46. doi: 10.1016/j.ijfoodmicro.2017.02.011. Epub 2017 Feb 21.
This work aimed to develop a nondairy fermented beverage from a blend of cassava and rice based on Brazilian indigenous beverage cauim using probiotic lactic acid bacteria (LAB) and yeast. The indigenous strains Lactobacillus plantarum CCMA 0743 (from cauim) and Torulaspora delbrueckii CCMA 0235 (from tarubá), and the commercial probiotic, L. acidophilus LAC-04, were used as starter cultures in single and co-cultivations. The bacteria populations were around 8.0 log (CFU/mL) at the end of all fermentations as recommended for probiotic products. Higher residual starch contents were noted in the single LAB cultures (10.6% [w/w]) than in co-cultures (<6% [w/w]), showing that co-culture may help the digestibility. For all different assays (single and co-culture), lactic acid was the main organic acid detected (>1.6g/L) and ethanol was lower than 0.5% (w/v) consisting in a non-alcoholic beverage. The assays containing yeast showed the highest antioxidant activity (around 10% by DPPH and ABTS methods). Therefore, a nondairy fermented beverage was successfully obtained, and the co-culture of LAB and T. delbrueckii could increase the product's functional properties.
本工作旨在利用益生菌乳酸菌(LAB)和酵母,从基于巴西本土饮料 cauim 的木薯和大米混合物中开发一种非乳制品发酵饮料。使用了本土菌株植物乳杆菌 CCMA 0743(来自 cauim)和毕赤酵母 CCMA 0235(来自 tarubá),以及商业益生菌嗜酸乳杆菌 LAC-04 作为单一和共培养的起始培养物。如推荐的益生菌产品一样,所有发酵结束时细菌种群数量均达到 8.0 log(CFU/mL)左右。在单一 LAB 培养物中(10.6%[w/w])残留淀粉含量高于共培养物(<6%[w/w]),表明共培养可能有助于消化。对于所有不同的测定(单一和共培养),乳酸是检测到的主要有机酸(>1.6g/L),乙醇含量低于 0.5%(w/v),这是一种非酒精饮料。含有酵母的测定显示出最高的抗氧化活性(DPPH 和 ABTS 方法约为 10%)。因此,成功获得了一种非乳制品发酵饮料,LAB 和 T. delbrueckii 的共培养可以提高产品的功能特性。