Department of Biology, Federal University of Lavras, 37.200-000 Lavras, MG, Brazil.
Department of Basic Science, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 39100-000, Diamantina, MG, Brazil.
Food Res Int. 2017 Oct;100(Pt 1):698-707. doi: 10.1016/j.foodres.2017.07.070. Epub 2017 Aug 2.
There is an important demand for the development of new non-dairy probiotic beverages in the functional food market. This work aimed to develop new fermented beverages from maize and rice. Lactobacillus plantarum CCMA 0743, Torulaspora delbrueckii CCMA 0235, and the commercial probiotic Lactobacillus acidophilus LACA 4, were used as a mixed starter culture. Two prebiotic concentrations, 20 and 50g/L fructooligosaccharide (FOS) were tested. The growth of L. acidophilus LACA 4 was favored by 50g/L FOS and after refrigerated storage at 4°C for 28days, its population remained above 10CFU/mL. Lactic and acetic acids were the main organic acids detected, at around 3.7 and 0.5g/L, respectively. Ethanol was present at <5g/L in non-alcoholic beverages. Fifty-five volatile compounds including acids, alcohols, aldehydes, esters, ketones, pyrazines and others, were detected. The sensorial analysis demonstrated that >50% of consumers liked slightly or liked extremely the beverages (scores from 6-9). Therefore, potential symbiotic cereal beverages were successfully obtained using a mix of lactic acid bacteria and yeast as a starter culture. This is an important step in the commercial production of alternative beverages from common food substrates for consumers.
在功能性食品市场上,人们对开发新的非乳制品益生菌饮料有很大的需求。本工作旨在从玉米和大米中开发新的发酵饮料。使用植物乳杆菌 CCMA 0743、毕赤酵母 CCMA 0235 和商业益生菌嗜酸乳杆菌 LACA 4 作为混合发酵剂。测试了两种益生元浓度,20 和 50g/L 低聚果糖(FOS)。50g/L FOS 有利于嗜酸乳杆菌 LACA 4 的生长,在 4°C 冷藏储存 28 天后,其数量仍保持在 10CFU/mL 以上。检测到的主要有机酸为乳酸和乙酸,分别约为 3.7 和 0.5g/L。非酒精饮料中乙醇含量<5g/L。检测到包括酸、醇、醛、酯、酮、吡嗪等在内的 55 种挥发性化合物。感官分析表明,超过 50%的消费者喜欢或非常喜欢这种饮料(评分在 6-9 之间)。因此,使用混合乳酸菌和酵母作为发酵剂成功获得了潜在的共生谷物饮料。这是消费者用常见食品基质进行替代饮料商业化生产的重要一步。