Ardestani Fatemeh
Department of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran.
Iran J Pharm Res. 2016 Fall;15(4):925-931.
Mycophenolic acid is a secondary extracellular metabolite of strains with numerous pharmaceutical properties such as antibiotic and immunosuppressive uses. The aim of this work is the survey of the effect of phenylglyoxal and n-ethylmaleimide concentration in culture medium on mycophenolic acid production by ATCC16024 was investigated. Batch submerged fermentation was performed in 250 mL shake flasks at 24 °C and 200 rpm in a rotary shaker for 300 h using a basic culture medium containing different concentrations of phenylglyoxal and n-ethylmaleimide ranged from 0 to 20 mg. L. For the basic medium without any amounts of phenylglyoxal and n-ethylmaleimide (control), maximum MPA production, product yield and productivity of process was in order, 1.5042 g. L, 20.3 mg. g consumed glucose and 5.37 mg. Lh. Maximum produced MPA of 2.9032 g. L, MPA yield of 39.23 mg. g of consumed glucose, productivity of 10.37 mg. L h and total enhancement of 93.11% was obtained when the culture medium was contained 18 mg. L of phenylglyoxal, represented more than 93% raising in compare to control. Maximum MPA concentration, yield and productivity in order was obtained 3.1123 g. L, 42.06 mg. g of consumed glucose and 11.11 mg. L h, with using 6 mg. L of n-ethylmaleimide. N-ethylmaleimide was caused to 2.138 folds (106.89%) increase in MPA production by ATCC16024.
霉酚酸是具有多种药学特性（如抗生素和免疫抑制用途）的菌株的次级细胞外代谢产物。本研究的目的是调查培养基中苯乙二醛和N - 乙基马来酰亚胺浓度对ATCC16024产霉酚酸的影响。在250 mL摇瓶中于24°C和200 rpm的旋转摇床上进行分批深层发酵300小时，使用含有浓度范围为0至20 mg/L的不同浓度苯乙二醛和N - 乙基马来酰亚胺的基础培养基。对于不含任何苯乙二醛和N - 乙基马来酰亚胺的基础培养基（对照），最大霉酚酸产量、产物得率和过程生产力依次为1.5042 g/L、20.3 mg/g消耗葡萄糖和5.37 mg/L·h。当培养基含有18 mg/L苯乙二醛时，获得最大产霉酚酸量为2.9032 g/L、霉酚酸得率为39.23 mg/g消耗葡萄糖、生产力为10.37 mg/L·h且总提高率为93.11%，与对照相比提高超过93%。使用6 mg/L的N - 乙基马来酰亚胺时，依次获得最大霉酚酸浓度、得率和生产力为3.1123 g/L、42.06 mg/g消耗葡萄糖和11.11 mg/L·h。N - 乙基马来酰亚胺使ATCC16024的霉酚酸产量增加2.138倍（106.89%）。
