Karki Surya B, Yildirim-Ayan Eda, Eisenmann Kathryn M, Ayan Halim
Department of Bioengineering, College of Engineering, University of Toledo, Toledo, OH 43606, USA.
Department of Bioengineering, College of Engineering, University of Toledo, Toledo, OH 43606, USA; Department of Orthopaedic Surgery, University of Toledo Medical Center, Toledo, OH 43614, USA.
Biomed Res Int. 2017;2017:8058307. doi: 10.1155/2017/8058307. Epub 2017 Jan 24.
Traditional cancer treatments like radiotherapy and chemotherapy have drawbacks and are not selective for killing only cancer cells. Nonthermal atmospheric pressure plasmas with dielectric barrier discharge (DBD) can be applied to living cells and tissues and have emerged as novel tools for localized cancer therapy. The purpose of this study was to investigate the different effects caused by miniature DBD (mDBD) plasma to A549 lung cancer cells. In this study, A549 lung cancer cells cultured in 12 well plates were treated with mDBD plasma for specified treatment times to assess the changes in the size of the area of cell detachment, the viability of attached or detached cells, and cell migration. Furthermore, we investigated an innovative mDBD plasma-based therapy for localized treatment of lung cancer cells through apoptotic induction. Our results indicate that plasma treatment for 120 sec causes apoptotic cell death in 35.8% of cells, while mDBD plasma treatment for 60 sec, 30 sec, or 15 sec causes apoptotic cell death in 20.5%, 14.1%, and 6.3% of the cell population, respectively. Additionally, we observed reduced A549 cell migration in response to mDBD plasma treatment. Thus, mDBD plasma system can be a viable platform for localized lung cancer therapy.
传统的癌症治疗方法，如放疗和化疗，存在缺陷，且并非仅对癌细胞具有选择性杀伤作用。具有介质阻挡放电（DBD）的非热大气压等离子体可应用于活细胞和组织，并已成为局部癌症治疗的新型工具。本研究的目的是调查微型DBD（mDBD）等离子体对A549肺癌细胞产生的不同影响。在本研究中，将培养在12孔板中的A549肺癌细胞用mDBD等离子体处理特定的时间，以评估细胞脱离区域大小的变化、贴壁或脱离细胞的活力以及细胞迁移情况。此外，我们研究了一种基于mDBD等离子体的创新疗法，通过诱导凋亡对肺癌细胞进行局部治疗。我们的结果表明，等离子体处理120秒会导致35.8%的细胞发生凋亡性细胞死亡，而mDBD等离子体处理60秒、30秒或15秒分别会导致20.5%、14.1%和6.3%的细胞群体发生凋亡性细胞死亡。此外，我们观察到mDBD等离子体处理后A549细胞迁移减少。因此，mDBD等离子体系统可以成为局部肺癌治疗的可行平台。
